Involvement of 14-3-3 proteins in the osmotic regulation of H+- ATPase in plant plasma membranes
| Autor: | A. H. de Boer, Oleg I. Klychnikov, A. V. Babakov, M. S. Trofimova, Vera V Chelysheva, S. E. Zorinyanz |
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| Přispěvatelé: | Developmental Genetics |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Osmotic shock
Tyrosine 3-Monooxygenase ATPase Turgor pressure Hypertonic Solutions Plant Science chemistry.chemical_compound ATP hydrolysis Genetics Homeostasis Sorbitol Cells Cultured Osmotic concentration biology Osmolar Concentration Hydrogen-Ion Concentration Proton-Translocating ATPases Membrane chemistry Biochemistry 14-3-3 Proteins Fusicoccin Osmoregulation biology.protein Plants Edible Dimerization |
| Zdroj: | Babakov, A V, Chelysheva, V V, Klychnikov, O L, Zorinyanz, S E, Trofimova, M S & de Boer, A H 2000, ' Involvement of 14-3-3 proteins in the osmotic regulation of H+-ATPase in plant plasma membranes. ', Planta, vol. 211, pp. 446-448 . https://doi.org/10.1007/s004250000347 Planta, 211, 446-448. Springer Verlag |
| ISSN: | 0032-0935 |
| DOI: | 10.1007/s004250000347 |
| Popis: | Taking the binding of fusicoccin to plasma membranes as an indicator of complex formation between the 14-3-3 dimer and H+-ATPase, we assessed the effect of osmotic stress on the interaction of these proteins in suspension-cultured cells of sugar beet (Beta vulgaris L.). An increase in osmolarity of the cell incubation medium, accompanied by a decrease in turgor, was found to activate the H+ efflux 5-fold. The same increment was observed in the number of high-affinity fusicoccin-binding sites in isolated plasma membranes; the 14-3-3 content in the membranes increased 2- to 3-fold, while the H+-ATPase activity changed only slightly. The data obtained indicate that osmotic regulation of H+-ATPase in the plant plasma membrane is achieved via modulation of the coupling between H+ transport and ATP hydrolysis, and that such regulation involves 14-3-3 proteins. |
| Databáze: | OpenAIRE |
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