The Effect of BML-111 in Preeclampsia Rat Model Induced by the Low Dose of Cadmium Chloride
| Autor: | Min Hu, Qiong Zhang, Yinping Huang, KeKe Zhang, Lin Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty H&E stain Cadmium chloride lcsh:Gynecology and obstetrics Preeclampsia preeclampsia 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot Internal medicine Placenta Medicine lcsh:RG1-991 Kidney medicine.diagnostic_test business.industry Therapeutic effect Obstetrics and Gynecology Interleukin BML-111 medicine.disease cadmium chloride 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry inflammation 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health Original Article business |
| Zdroj: | AJP Reports American Journal of Perinatology Reports, Vol 09, Iss 03, Pp e201-e208 (2019) |
| ISSN: | 2157-7005 2157-6998 |
| DOI: | 10.1055/s-0039-1693016 |
| Popis: | Aim This article determines the optimal time and dose of cadmium chloride (CdCl2) injected to pregnant rat to establish experimental preeclampsia (PE) model. In addition, the therapeutic potential of BML-111, a lipoxin A4 analogue, in the CdCl2-induced PE model was also evaluated. Methods Peritoneal injection of two dose of CdCl2 for successive 6 days was tested in the pregnant rats starting from various gestational days (GDs). During this process, the systolic blood pressure and the body weight of pregnant rats and neonatal rats were monitored. The pathological changes of the placenta and kidney were evaluated by hematoxylin and eosin staining. The phosphorylation of extracellular signal-regulated kinase 1/2 and signal transducer and activator of transcription 3 in the placentas was detected by Western blot, and the messenger ribonucleic acid expression of interleukin (IL)-6, tumor necrosis factor-α, and IL-10 in the placentas were detected by real-time polymerase chain reaction. BML-111 at the dose of 1 mg/kg/day was peritoneally injected into the rat after establishing the PE model to test its therapeutic potential. Results In the present study, we successfully established the PE model in pregnant rats by intraperitoneally injection of CdCl2 at the dose of 0.125 mg/kg/day from GD 9 to 14. We recapitulated multiple features of clinical PE in CdCl2-induced rat, including high blood pressure, renal dysfunction, and inflammatory response in placenta. Furthermore, treatment with BML-111 significantly relieved multiple features in our PE rat model. Conclusions BML-111 has a potential therapeutic effect in pregnant rats with CdCl2-induced PE, which appears to be mediated through inhibition of inflammatory processes in the placenta. |
| Databáze: | OpenAIRE |
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