High-dose cytostatic agents in allogeneic bone marrow transplantation: comparison of the engraftment promoting potential

Autor: Winfried Gassmann, Norbert Schmitz, Helmut Löffler, B. Glass, W. Mueller-Ruchholtz, L. Uharek, A. Erbersdobler, H.-U. Wottge
Rok vydání: 1991
Předmět:
Zdroj: British Journal of Haematology. 78:310-318
ISSN: 1365-2141
0007-1048
DOI: 10.1111/j.1365-2141.1991.tb04442.x
Popis: Summary. We investigated the potential of various cytostatic agents for preventing graft rejection following allogeneic bone marrow transplantation. LEW rats received a lethal dose (35 mg/kg) of busulfan followed by injection of 1 x 108 F1(CAP x LEW) marrow cells, which are unable to induce a graft-versus-host reaction in LEW recipients. Rejection of the marrow graft was assessed by monitoring haematocrit and granulocyte counts. Due to its weak immunosuppressive activity, busulfan by itself is unable to allow engraftment of allogeneic marrow. Therefore, agents administered in addition to busulfan can be tested for their capacity to prevent marrow graft rejection. 120 mg/kg of cyclophosphamide, 20 mg/kg of ACNU and 240 mg/kg of ifosfamide completely prevented rejection of the allogeneic marrow. Maximum doses of BCNU applicable in conjunction with busulfan reduced the rejection rate to 12% (30 mg/kg) and 17% (40 mg/kg), whereas the antitumour agents thiotepa, melphalan, and carboplatin exhibited a very limited engraftment-promoting potential in this experimental setting. Thus, BCNU (carmustine), ACNU (nimustine), and ifosfamide might be suitable candidates for conditioning of allogeneic bone marrow graft recipients.
Databáze: OpenAIRE
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