Expression of the Epstein-Barr virus DNA polymerase inEscherichia coli for use as antigen for the diagnosis of nasopharyngeal carcinoma
| Autor: | Wu-Tse Liu, Lung-Shen Lin, Kuang Y. Chen, Huey-Lang Yang, Chih-Hung Shu, Wan-Ling Huang, Lee-Hwa Ro, Mu-Shung Lo, Tong-Hsuan Chang, Kuan-Chih Chow, Jeng Ma |
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| Rok vydání: | 1995 |
| Předmět: |
Herpesvirus 4
Human DNA polymerase viruses Blotting Western Molecular Sequence Data Cytomegalovirus Gene Expression DNA-Directed DNA Polymerase Herpesvirus 1 Human Cross Reactions Antibodies Viral medicine.disease_cause Sensitivity and Specificity Virus Serology law.invention Viral Proteins Western blot law Virology Escherichia coli medicine Humans Amino Acid Sequence Cloning Molecular Antigens Viral Polymerase Base Sequence biology medicine.diagnostic_test Carcinoma Nasopharyngeal Neoplasms Epstein–Barr virus Molecular biology Recombinant Proteins DNA-Binding Proteins Infectious Diseases Herpes simplex virus Immunoglobulin G biology.protein Recombinant DNA |
| Zdroj: | Journal of Medical Virology. 45:99-105 |
| ISSN: | 1096-9071 0146-6615 |
| Popis: | Epstein-Barr virus (EBV) encoded DNA polymerase (POL) was cloned and over-expressed in Escherichia coli. Western blot analysis confirmed the presence of antibody to this POL protein in sera from nasopharyngeal carcinoma (NPC) patients. By Western blot analysis, moderate to high concentration of IgG POL-specific antibodies were present in 43 of 48 NPC sera and only 4 of 48 healthy, seropositive controls. The POL-specific IgG antibodies appear as early as stage I of NPC, suggesting that the recombinant POL protein can be a useful diagnostic marker for early diagnosis of the disease. It was also found that human sera containing high titer of cytomegalovirus (CMV) antibodies or herpes simplex virus type 1 (HSV-1) antibodies did not cross-react with the recombinant EBV POL, despite the homology shared by DNA polymerase proteins of these viruses. © 1995 Wiiey-Liss, inc. |
| Databáze: | OpenAIRE |
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