Gastroprotective effect of oral kefir on indomethacin-induced acute gastric lesions in mice: Impact on oxidative stress
| Autor: | Gisele Maziero Alves, Thiago de Melo Costa Pereira, Silvana S. Meyrelles, Larissa Zambom Côco, Beatriz Peters, Elisardo C. Vasquez, Kelly Ribeiro Moura Barboza, Bianca P. Campagnaro |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Antioxidant medicine.medical_treatment Indomethacin Lansoprazole Administration Oral Oxidative phosphorylation Pharmacology medicine.disease_cause Protective Agents General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Mice Kefir medicine Gastric mucosa Animals Stomach Ulcer General Pharmacology Toxicology and Pharmaceutics biology Superoxide Anti-Inflammatory Agents Non-Steroidal General Medicine Anti-Ulcer Agents Oxidative Stress 030104 developmental biology medicine.anatomical_structure chemistry Acute Disease biology.protein Cyclooxygenase Peroxynitrite Oxidative stress medicine.drug |
| Zdroj: | Life sciences. 209 |
| ISSN: | 1879-0631 |
| Popis: | Aims This study investigated the gastroprotective effects and the systemic oxidative status of oral kefir pretreatment in albino mice submitted to acute gastric ulcer induced by indomethacin. Main methods Male Swiss mice were divided into three groups (n = 7): Vehicle (0.3 mL of whole milk/100 g body weight, pH adjusted to 5.0), Kefir (0.3 mL of kefir/100 g body weight) and Proton Pump Inhibitor (PPI, 30 mg/kg of lansoprazole), via gavage for 14 days. Animals were fasted for 16 h and treated orally with indomethacin (40 mg/kg). After 6 h the animals were euthanized, the blood samples were obtained and used for the determination of ROS production, oxidation of macromolecules and apoptosis. The stomachs were removed, opened by the greater curvature, and a macroscopic analysis of the gastric lesions was performed. Key findings Our findings demonstrated that the symbiotic kefir significantly alleviated blood oxidative stress by reducing superoxide anion, hydrogen peroxide and hydroxyl/peroxynitrite radicals, thereby leading to reduced oxidative damage to macromolecules due to a decreased oxidative stress status in induced gastric lesions. These anti-oxidative properties might contribute favorably to the ulcer attenuation in the kefir group. Significance Taken together, these findings support a significant role played by the antioxidant actions of kefir in counteracting the gastric damage induced by this cyclooxygenase inhibitor. It is also worthy to mention that, kefir also exerted the gastroprotective property partly by inhibiting oxidative systemic damage. Based on these considerations, it was implied that kefir might be a contributor for the ROS-scavenging effect. |
| Databáze: | OpenAIRE |
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