PlcRa, a new quorum-sensing regulator from Bacillus cereus, plays a role in oxidative stress responses and cysteine metabolism in stationary phase
| Autor: | Samira Makhzami, Didier Lereclus, Watanalai Panbangred, Marcel H. Tempelaars, Isabelle Martin-Verstraete, Gwenaëlle André-Leroux, Ludovic Bridoux, Tjakko Abee, Pagakrong Wanapaisan, Eugénie Huillet |
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| Přispěvatelé: | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratory of Food Microbiology, Wageningen University and Research [Wageningen] (WUR), Unité Mathématique, Informatique et Génome (MIG), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Department of Biotechnology, Mahidol University [Bangkok], Cellule Pasteur UPMC, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris], Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes (PBA (U-Pasteur_6)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7), Wageningen University and Research Centre [Wageningen] (WUR), Unité Mathématique Informatique et Génome (MIG), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Huillet, Eugénie |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Transcription Genetic Operon Microarrays Mutant Regulator Gene Identification and Analysis Gene Expression lcsh:Medicine Levensmiddelenmicrobiologie virulence regulon chemistry.chemical_compound iii pantothenate kinase Biochemical Simulations Bacterial Physiology Promoter Regions Genetic lcsh:Science cell-cell communication 0303 health sciences Multidisciplinary quorum sensing subtilis Bacterial Pathogens DNA-Binding Proteins Biochemistry gram-positive bacteria Research Article Molecular Sequence Data Cystine binding protein Biology Microbiology Molecular Genetics global regulator 03 medical and health sciences Bacterial Proteins picra bacillus cereus Gene Regulation Amino Acid Sequence Cysteine Cysteine metabolism VLAG 030304 developmental biology Gram Positive 030306 microbiology lcsh:R Wild type Computational Biology Bacteriology Gene Expression Regulation Bacterial gene-expression Molecular biology toxin gene Oxidative Stress Regulon chemistry anthracis Mutation Food Microbiology Trans-Activators lcsh:Q |
| Zdroj: | PLoS ONE, Vol 7, Iss 12, p e51047 (2012) PLoS ONE PLoS ONE, Public Library of Science, 2012, 7, online (12), Non paginé. ⟨10.1371/journal.pone.0051047⟩ PLoS ONE, 2012, 7, online (12), Non paginé. ⟨10.1371/journal.pone.0051047⟩ PLoS ONE 7 (2012) 12 PLoS ONE, 7(12) Plos One 12 (7, online), Non paginé. (2012) |
| ISSN: | 1932-6203 |
| Popis: | Chantier qualité GA; We characterized a new quorum-sensing regulator, PlcRa, which is present in various members of the B. cereus group and identified a signaling heptapeptide for PlcRa activity: PapRa(7). We demonstrated that PlcRa is a 3D structural paralog of PlcR using sequence analysis and homology modeling. A comparison of the transcriptomes at the onset of stationary phase of a ΔplcRa mutant and the wild-type B. cereus ATCC 14579 strain showed that 68 genes were upregulated and 49 genes were downregulated in the ΔplcRa mutant strain (>3-fold change). Genes involved in the cysteine metabolism (putative CymR regulon) were downregulated in the ΔplcRa mutant strain. We focused on the gene with the largest difference in expression level between the two conditions, which encoded -AbrB2- a new regulator of the AbrB family. We demonstrated that purified PlcRa bound specifically to the abrB2 promoter in the presence of synthetic PapRa(7), in an electrophoretic mobility shift assay. We further showed that the AbrB2 regulator controlled the expression of the yrrT operon involved in methionine to cysteine conversion. We found that the ΔplcRa mutant strain was more sensitive to hydrogen peroxide- and disulfide-induced stresses than the wild type. When cystine was added to the culture of the ΔplcRa mutant, challenged with hydrogen peroxide, growth inhibition was abolished. In conclusion, we identified a new RNPP transcriptional regulator in B. cereus that activated the oxidative stress response and cysteine metabolism in transition state cells. |
| Databáze: | OpenAIRE |
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