Site-selective protein modification via disulfide rebridging for fast tetrazine/trans-cyclooctene bioconjugation
| Autor: | Seah Ling Kuan, Lujuan Xu, Tanja Weil, João C. F. Nogueira, Vijay Chudasama, Marco Raabe, Maksymilian Marek Zegota |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular 010402 general chemistry 01 natural sciences Biochemistry Tetrazine chemistry.chemical_compound Cyclooctanes Cyclooctene Heterocyclic Compounds Humans Disulfides Physical and Theoretical Chemistry Protein secondary structure Bioconjugation Molecular Structure 010405 organic chemistry Organic Chemistry Glutathione Combinatorial chemistry 0104 chemical sciences chemistry Reagent Immunoglobulin G Posttranslational modification Protein Processing Post-Translational Conjugate |
| Zdroj: | Organic & Biomolecular Chemistry |
| Popis: | An inverse electron demand Diels–Alder reaction between tetrazine and trans-cyclooctene (TCO) holds great promise for protein modification and manipulation. Herein, we report the design and synthesis of a tetrazine-based disulfide rebridging reagent, which allows the site-selective installation of a tetrazine group into disulfide-containing peptides and proteins such as the hormone somatostatin (SST) and the antigen binding fragment (Fab) of human immunoglobulin G (IgG). The fast and efficient conjugation of the tetrazine modified proteins with three different TCO-containing substrates to form a set of bioconjugates in a site-selective manner was successfully demonstrated for the first time. Homogeneous, well-defined bioconjugates were obtained underlining the great potential of our method for fast bioconjugation in emerging protein therapeutics. The formed bioconjugates were stable against glutathione and in serum, and they maintained their secondary structure. With this work, we broaden the scope of tetrazine chemistry for site-selective protein modification to prepare well-defined SST and Fab conjugates with preserved structures and good stability under biologically relevant conditions. |
| Databáze: | OpenAIRE |
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