Simultaneous assessment of lipid classes and bile acids in human intestinal fluid by solid-phase extraction and HPLC methods
| Autor: | Lars Löfgren, Eva M. Persson, Hans Lennernäs, Bertil Abrahamsson, Ralf Nilsson, Göran F. H. Hansson |
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| Rok vydání: | 2006 |
| Předmět: |
QD415-436
Biochemistry High-performance liquid chromatography Intestinal fluid Bile Acids and Salts chemistry.chemical_compound Acetic acid Endocrinology neutral lipids Lipolysis Humans Scattering Radiation solid-phase extraction Solid phase extraction high-performance liquid chromatography phospholipids Chromatography High Pressure Liquid evaporative light scattering Chromatography Elution Extraction (chemistry) Cell Biology Lipids Body Fluids Intestines chemistry lipids (amino acids peptides and proteins) Methanol |
| Zdroj: | Journal of Lipid Research, Vol 48, Iss 1, Pp 242-251 (2007) |
| ISSN: | 0022-2275 |
| Popis: | The purpose of the study reported here was to develop a method for the determination of lipid classes in intestinal fluids, including bile acids (BAs). A solid-phase extraction (SPE) method using C18 and silica columns for the separation of BAs, phospholipids (PLs), and neutral lipids (NLs), including free fatty acids, has been developed and validated. Fed-state small intestinal fluid collected from humans was treated with orlistat to inhibit lipolysis and mixed with acetic acid and methanol before SPE to maximize lipid recoveries. BAs, PLs, and NLs were isolated using lipophilic and polar solvents to promote elution from the SPE columns. The different lipid classes were subsequently analyzed using three separately optimized HPLC methods with evaporative light-scattering detectors. High recoveries (>90%) of all lipids evaluated were observed, with low coefficients of variation ( |
| Databáze: | OpenAIRE |
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