Natural variations of TFIIAγ gene and LOB1 promoter contribute to citrus canker disease resistance in Atalantia buxifolia

Autor: Xiaolin Jiang, Meng Yuan, Ling Ma, Ping Yin, Xia Wang, Xiuxin Deng, Qiang Xu, Yue Huang, Muhammad Junaid Rao, Yuantao Xu, Xiaomei Tang
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
Cancer Research
Citrus
Leaves
Gene Expression
Plant Science
QH426-470
01 natural sciences
Biochemistry
Xanthomonas citri
Gene Expression Regulation
Plant
Microbial Physiology
Gene expression
Promoter Regions
Genetic
Genetics (clinical)
Flowering Plants
Disease Resistance
Plant Proteins
Genetics
0303 health sciences
biology
Effector
Plant Anatomy
Microbial Growth and Development
Eukaryota
food and beverages
Plants
Experimental Organism Systems
Citrus canker
Research Article
Protein Binding
Nicotiana
Xanthomonas
Plant disease resistance
Research and Analysis Methods
Oranges
Microbiology
Fruits
03 medical and health sciences
Plant and Algal Models
DNA-binding proteins
medicine
Gene Regulation
Grasses
Molecular Biology
Gene
Rutaceae
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Plant Diseases
Canker
Bacterial Growth
Organisms
Biology and Life Sciences
Proteins
biology.organism_classification
medicine.disease
Regulatory Proteins
Transcription Factor TFIIA
Mutation
Animal Studies
Rice
010606 plant biology & botany
Transcription Factors
Developmental Biology
Zdroj: PLoS Genetics, Vol 17, Iss 1, p e1009316 (2021)
PLoS Genetics
ISSN: 1553-7404
1553-7390
Popis: Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating diseases in citrus industry worldwide. Most citrus cultivars such as sweet orange are susceptible to canker disease. Here, we utilized wild citrus to identify canker-resistant germplasms, and found that Atalantia buxifolia, a primitive (distant-wild) citrus, exhibited remarkable resistance to canker disease. Although the susceptibility gene LATERAL ORGAN BOUNDARIES 1 (LOB1) could also be induced in Atalantia after canker infection, the induction extent was far lower than that in sweet orange. In addition, three of amino acids encoded by transcription factor TFIIAγ in Atalantia (AbTFIIAγ) exhibited difference from those in sweet orange (CsTFIIAγ) which could stabilize the interaction between effector PthA4 and effector binding element (EBE) of LOB1 promoter. The mutation of AbTFIIAγ did not change its interaction with transcription factor binding motifs (TFBs). However, the AbTFIIAγ could hardly support the LOB1 expression induced by the PthA4. In addition, the activity of AbLOB1 promoter was significantly lower than that of CsLOB1 under the induction by PthA4. Our results demonstrate that natural variations of AbTFIIAγ and effector binding element (EBE) in the AbLOB1 promoter are crucial for the canker disease resistance of Atalantia. The natural mutations of AbTFIIAγ gene and AbLOB1 promoter in Atalantia provide candidate targets for improving the resistance to citrus canker disease.
Author summary It has been well documented that most citrus cultivars are susceptible to canker disease, while little is known about the resistance or susceptibility of primitive or wild citrus to canker disease. This study reveals that primitive citrus (Atalantia buxifolia) is highly resistant to citrus canker. Transcriptome data demonstrated that Atalantia had an active resistance response to the infection of Xcc, compared with susceptible sweet orange. Our results indicated that natural variations of AbTFIIAγ gene and AbLOB1 promoter contributed to the resistance. Hence, we propose that the natural mutations of AbTFIIAγ gene and AbLOB1 promoter could provide candidate targets for breeding canker resistant citrus.
Databáze: OpenAIRE
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