Natural variations of TFIIAγ gene and LOB1 promoter contribute to citrus canker disease resistance in Atalantia buxifolia
Autor: | Xiaolin Jiang, Meng Yuan, Ling Ma, Ping Yin, Xia Wang, Xiuxin Deng, Qiang Xu, Yue Huang, Muhammad Junaid Rao, Yuantao Xu, Xiaomei Tang |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Cancer Research Citrus Leaves Gene Expression Plant Science QH426-470 01 natural sciences Biochemistry Xanthomonas citri Gene Expression Regulation Plant Microbial Physiology Gene expression Promoter Regions Genetic Genetics (clinical) Flowering Plants Disease Resistance Plant Proteins Genetics 0303 health sciences biology Effector Plant Anatomy Microbial Growth and Development Eukaryota food and beverages Plants Experimental Organism Systems Citrus canker Research Article Protein Binding Nicotiana Xanthomonas Plant disease resistance Research and Analysis Methods Oranges Microbiology Fruits 03 medical and health sciences Plant and Algal Models DNA-binding proteins medicine Gene Regulation Grasses Molecular Biology Gene Rutaceae Ecology Evolution Behavior and Systematics 030304 developmental biology Plant Diseases Canker Bacterial Growth Organisms Biology and Life Sciences Proteins biology.organism_classification medicine.disease Regulatory Proteins Transcription Factor TFIIA Mutation Animal Studies Rice 010606 plant biology & botany Transcription Factors Developmental Biology |
Zdroj: | PLoS Genetics, Vol 17, Iss 1, p e1009316 (2021) PLoS Genetics |
ISSN: | 1553-7404 1553-7390 |
Popis: | Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating diseases in citrus industry worldwide. Most citrus cultivars such as sweet orange are susceptible to canker disease. Here, we utilized wild citrus to identify canker-resistant germplasms, and found that Atalantia buxifolia, a primitive (distant-wild) citrus, exhibited remarkable resistance to canker disease. Although the susceptibility gene LATERAL ORGAN BOUNDARIES 1 (LOB1) could also be induced in Atalantia after canker infection, the induction extent was far lower than that in sweet orange. In addition, three of amino acids encoded by transcription factor TFIIAγ in Atalantia (AbTFIIAγ) exhibited difference from those in sweet orange (CsTFIIAγ) which could stabilize the interaction between effector PthA4 and effector binding element (EBE) of LOB1 promoter. The mutation of AbTFIIAγ did not change its interaction with transcription factor binding motifs (TFBs). However, the AbTFIIAγ could hardly support the LOB1 expression induced by the PthA4. In addition, the activity of AbLOB1 promoter was significantly lower than that of CsLOB1 under the induction by PthA4. Our results demonstrate that natural variations of AbTFIIAγ and effector binding element (EBE) in the AbLOB1 promoter are crucial for the canker disease resistance of Atalantia. The natural mutations of AbTFIIAγ gene and AbLOB1 promoter in Atalantia provide candidate targets for improving the resistance to citrus canker disease. Author summary It has been well documented that most citrus cultivars are susceptible to canker disease, while little is known about the resistance or susceptibility of primitive or wild citrus to canker disease. This study reveals that primitive citrus (Atalantia buxifolia) is highly resistant to citrus canker. Transcriptome data demonstrated that Atalantia had an active resistance response to the infection of Xcc, compared with susceptible sweet orange. Our results indicated that natural variations of AbTFIIAγ gene and AbLOB1 promoter contributed to the resistance. Hence, we propose that the natural mutations of AbTFIIAγ gene and AbLOB1 promoter could provide candidate targets for breeding canker resistant citrus. |
Databáze: | OpenAIRE |
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