Development of a model system for evaluation of human endothelial cell proliferationin Vitro: Possible clinical applications
| Autor: | A. M. Olfer'ev, I. E. Petrichenko, N. V. Koval'chuk, V. A. Metel'skaya, G. N. Shchukina, S. A. Popkov |
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| Rok vydání: | 2000 |
| Předmět: |
Drug
Medroxyprogesterone medicine.medical_specialty Hormone Replacement Therapy Angiogenesis media_common.quotation_subject Cell Culture Techniques Model system General Biochemistry Genetics and Molecular Biology Blood serum Internal medicine Humans Medicine Hormone replacement therapy media_common Estradiol business.industry General Medicine In vitro Endothelial stem cell Drug Combinations Endocrinology Female Endothelium Vascular Menopause business Cell Division medicine.drug |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 129:342-345 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | A system for evaluation of the ability of human blood serum to affect endothelial cell proliferation was developed and tested. The system based on incorporation of (3)H-thymidine into DNA was used to analyze the effects of hormone replacement therapy on endothelial repair and angiogenesis. Blood serum from 12 menopausal women less effectively activated endothelial proliferation compared to control donor serum. After 6-month hormone replacement therapy with Divina (a combination of estradiol and medroxyprogesterone), this index increased in seven female subjects (58.3%), but remained below the control level. The model proposed by us can be used in clinical practice and drug testing for evaluation of the influence of blood serum on vascular endothelium. |
| Databáze: | OpenAIRE |
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