Ameliorative effect of the probiotic peptide against benzo(α)pyrene-induced inflammatory damages in enterocytes

Autor:	Min Luo, Dan Luo, Jie Liu, Huailing Wang, Xiaoyu Liu, Min Yang, Fangfang Tian, Suofu Qin, Yuying Li
Rok vydání:	2022
Předmět:	Pharmacology NF-E2-Related Factor 2 Probiotics Immunology Quinones NAD Peptides Cyclic Mice Disease Models Animal Enterocytes Receptors Aryl Hydrocarbon Benzo(a)pyrene Humans Animals Cytokines Immunology and Allergy Caco-2 Cells Reactive Oxygen Species Heme Oxygenase-1
Zdroj:	International Immunopharmacology. 112:109255
ISSN:	1567-5769
DOI:	10.1016/j.intimp.2022.109255
Popis:	Probiotics are living bacteria that provide health benefits to the host when consumed in sufficient quantities. However, the protective effect of the bioactive peptides isolated from the probiotics against benzo(α)pyrene (BaP) induced gastrointestinal injury has never been investigated. The current work used a bio-assay guided technique to identify-four new cyclic peptides in BaP-induced Caco-2 cell culture and mouse colitis model. Lactobacillus rhamnosus cycle (Thr-His-Ala-Trp) peptide-1 (LRCP-1) effectively inhibited BaP-induced epithelial cytokine over-release and intracellular ROS over-production. Simultaneously, LRCP-1 attenuated BaP-induced NAD (P)H: oxidases (NOXs), Matrix metalloproteinases (MMPs) over-expression, respectively. Furthermore, increased NAD (P)H: quinone oxidoreductase 1 (NQO1)/heme oxygenase-1 (HO-1)/nuclear factor E2-related factor 2 (Nrf2) expression and aryl hydrocarbon receptor (AhR) pathway activation induced by the BaP-exposure were also inhibited after the LRCP-1 treatment. Notably, LRCP-1 is a promising agent protecting gastrointestinal epithelial cells from BaP-induced inflammatory and oxidative damages.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a55e817a2188714ab2213fdec165211 https://doi.org/10.1016/j.intimp.2022.109255 Zobrazit plný text záznamu Full Text from ScienceDirect