Phytochrome-induced expression of lig1 , a homologue of the fission yeast cell-cycle checkpoint gene hus1 , is associated with the developmental switch in Physarum polycephalum plasmodia
| Autor: | Anthony R. Cashmore, Wolfgang Marwan, Roland Kroneder |
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| Rok vydání: | 1999 |
| Předmět: |
Spores
Transcriptional Activation Light Genes Protozoan Molecular Sequence Data Protozoan Proteins Cell Cycle Proteins Physarum polycephalum Schizosaccharomyces Genetics Homologous chromosome Animals Amino Acid Sequence RNA Messenger Gene chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid biology Phytochrome Reverse Transcriptase Polymerase Chain Reaction Cell Cycle Gene Expression Regulation Developmental Cell Differentiation General Medicine Cell cycle biology.organism_classification Amino acid Cell biology chemistry Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Sequence Alignment RNA Protozoan Function (biology) |
| Zdroj: | Current Genetics. 36:86-93 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/s002940050476 |
| Popis: | Lig1 was found in a differential-display screen for early genes expressed during phytochrome-controlled sporulation of Physarum polycephalum plasmodia. A stretch of 218 amino acids of the predicted sequence of Lig1 shares 32% sequence identity to that of the Schizosaccharomyces pombe cell-cycle and DNA-damage checkpoint gene hus1. In addition Lig1 is homologous to proteins of unknown function in Homo sapiens (35% identity) and Mus musculus (31% identity). Induction of lig1 expression was found to be controlled downstream from the point of integration of the phytochrome-activated pathway and the pathway sensing the metabolic state, but upstream of the developmental switch. The lig1 expression level in individual plasmodia correlated positively with the probability to sporulate. Sensory control of the lig1 expression level and its association with the developmental switch suggests a possible mechanism for the coordination of differentiation and the control of cell-cycle progression during the sporulation of P. polycephalum. |
| Databáze: | OpenAIRE |
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