Time course study of L-tyrosine aminotransferase induction in rat liver cell lines
| Autor: | Prudent Padieu, Martine Chessebeuf, Michel Fischbach |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
medicine.medical_specialty
Health Toxicology and Mutagenesis Toxicology Dexamethasone Epithelium Cell Line Basal (phylogenetics) Tyrosine aminotransferase Internal medicine Mole medicine Animals Enzyme inducer Tyrosine Transaminase chemistry.chemical_classification biology Cell Biology Molecular biology Rats Enzyme medicine.anatomical_structure Endocrinology Liver chemistry Cell culture Enzyme Induction biology.protein medicine.drug |
| Zdroj: | Cell Biology and Toxicology. 1:31-40 |
| ISSN: | 1573-6822 0742-2091 |
| Popis: | The enhancement of L-tyrosine aminotransferase activity by dexamethasone, an exclusive function of the liver, was serially measured at different passages of eight rat liver epithelial cell lines initiated and continuously grown in either a serum-supplemented medium or a serum-free medium. The enzyme basal activity was found to be 5.4 +/- 1.8 mU for cell lines in serum and 6.8 +/- 3.4 mU for cell lines without serum. Under the influence of dexamethasone (10(-6) mol/l for 5 hours) this basal level could be increased up to 2.9 fold in the presence of serum and 2.5 fold in its absence when investigations were carried out at early passages. During the following subcultures the induction ratio gradually declined and scarcely any induction could be detected after the 15th passage for cells grown in serum and after the 25th passage for cell lines grown without serum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink