The integration of HR-HPV increases the expression of cyclins A and E in cytologies with and without low-grade lesions
| Autor: | Berenice Illades-Aguiar, Eugenia Flores-Alfaro, Ma Isabel Zubillaga-Guerrero, José Francisco Muñoz-Valle, Marco Antonio Leyva-Vázquez, Eduardo Castañeda-Saucedo, Luz del Carmen Alarcón-Romero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Cyclin A In situ hybridization Pathology and Forensic Medicine law.invention Cyclin-A cyclin-E high-risk human papillomavirus in situ hybridization low-grade squamous intraepithelial lesion law medicine lcsh:QH573-671 Genotyping Polymerase chain reaction Cyclin Cervical cancer biology lcsh:Cytology virus diseases medicine.disease Molecular biology female genital diseases and pregnancy complications Squamous intraepithelial lesion biology.protein Original Article Restriction fragment length polymorphism |
| Zdroj: | Journal of Cytology, Vol 30, Iss 1, Pp 1-7 (2013) Journal of Cytology / Indian Academy of Cytologists |
| ISSN: | 0970-9371 |
| Popis: | Background: Cyclin-A and cyclin-E are regulators of G1-S phase of normal cell cycle. Integration of human papilloma virus high-risk (HR-HPV) could alter this mechanism, and its overexpression has been associated with poor prognosis in cervical cancer. Aim: To determine the expression of cyclin-A and cyclin-E, types of HR-HPV and physical state of DNA in cytologies with the diagnosis of low-grade squamous intraepithelial lesion (LSIL). Materials and Methods: 115 cytological specimens in liquid base (liquid-PREP™ ) were analyzed. 25 specimens were with no signs of SIL (NSIL) and without HPV; 30 with NSIL with low-risk HPV (LR-HPV); 30 with NSIL with HR-HPV; and 30 with both LSIL and HR-HPV. The expression of cyclins was evaluated by immunocytochemistry; and the detection of viral DNA was done by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLPs) for genotyping or sequencing of HPV. The physical state of HPV was evaluated by in situ hybridization with amplification with tyramide. Results: In the cytologies NSIL with LR-HPV, the expression of cyclin-A and cyclin-E was found respectively in 23.3% and 33.3% of the specimens. Among the specimens of NSIL with HR-HPV, 33.3% expressed cyclin-A and 40% cyclin-E, while 100% of the LSILs expressed the 2 cyclins. On the other hand, 100% of the samples NSIL with LR-HPV presented an episomal pattern. Of the specimens of NSIL with HR-HPV, 56.6% exhibited an episomal pattern, 23.3% integrated and 20%, mixed. Among the LSILs, 90% were mixed and 10% integrated. Conclusions: The cyclins A and E are present in the LSILs that occur predominantly in mixed state in the presence of HR-HPV. |
| Databáze: | OpenAIRE |
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