Lentivirus carrying the NeuroD1 gene promotes the conversion from glial cells into neurons in a spinal cord injury model
| Autor: | Bao-Quan Zhang, Shu-Xia Xu, Wen-Hao Chen, Ren Lin, Wei Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Endogeny Nerve Tissue Proteins Biology Group A Andrology 03 medical and health sciences Neural Stem Cells In vivo medicine Basic Helix-Loop-Helix Transcription Factors Animals Rats Wistar Spinal cord injury Spinal Cord Injuries Neurons General Neuroscience Lentivirus Spinal cord medicine.disease Neural stem cell Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Spinal Cord Immunology Immunohistochemistry Neuron Neuroglia |
| Zdroj: | Brain research bulletin. 135 |
| ISSN: | 1873-2747 |
| Popis: | In this study, we aimed to reprogram reactive glial cells into neural stem cells and neurons in vivo, by up-regulating NeuroD1. 60 rats were randomly divided into three groups with equal numbers. All rats received a spinal cord injury. Group A received only normal saline injection. Group B received unloaded lentivirus injection. Group C received NeuroD1 recombinant lentivirus injection. The BBB scores of the animals were documented at 3, 7, 14, 21days after SCI. 5 random rats of each group were removed at those timings after SCI and sacrificed. In the spinal cord slices, the numbers and morphology of neural stem cells, immature neurons and mature neurons were examined after immunohistochemical staining. Except day 3 after SCI, the BBB scores of group C on days 7, 14 and 21 were significantly higher than the other two groups (P |
| Databáze: | OpenAIRE |
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