Real-time PCR quantitation of FE65 a beta-amyloid precursor protein-binding protein after traumatic brain injury in rats
| Autor: | Masato Nakatome, Ryoji Matoba, Morio Iino, Hiromasa Inoue, Tasuku Fujii, Yukiko Ino, Hisanaga Kuroki, Harutoshi Fujimura, Toshiyuki Terao, Yoshiaki Ogura |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Traumatic brain injury Enolase Autopsy Diffuse Axonal Injury Nerve Tissue Proteins Pathology and Forensic Medicine Rats Sprague-Dawley Immunolabeling Amyloid beta-Protein Precursor medicine Animals Humans RNA Messenger Messenger RNA business.industry Reverse Transcriptase Polymerase Chain Reaction Binding protein Diffuse axonal injury Nuclear Proteins medicine.disease Rats Real-time polymerase chain reaction business Biomarkers Protein Binding |
| Zdroj: | International journal of legal medicine. 117(3) |
| ISSN: | 0937-9827 |
| Popis: | In cases of traumatic brain injury (TBI) in which the patient survived for only a short period of time and was without macroscopic changes at autopsy, it is difficult to diagnose TBI. To detect early diagnostic markers of diffuse axonal injury (DAI), real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in an experimental head trauma model of rat was chosen. The beta-amyloid precursor protein (beta-APP) is a well-known diagnostic marker of DAI which can be detected by immunolabeling as early as 1.5 h after injury. beta-APP has a binding protein, FE65, which is expressed in the brain of Alzheimer's disease patients along with beta-APP, but no involvement with brain injury has been reported. Neuron-specific enolase (NSE) is also a useful marker of DAI. We found that FE65 expression increased dramatically as early as 30 min after injury and decreased after peaking 1 h post-injury, although NSE showed no significant changes. These results suggest that real-time PCR of FE65 mRNA is useful for the diagnosis of DAI in forensic cases. |
| Databáze: | OpenAIRE |
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