Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks
| Autor: | José Ramón Botella, Michael G. Mason |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Time Factors Chromatography Bacteria Chemistry Dipstick Plants urologic and male genital diseases General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Nucleic Acids Viruses parasitic diseases Nucleic acid Animals Humans Cellulose Nucleic Acid Amplification Techniques 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Nature Protocols. 15:3663-3677 |
| ISSN: | 1750-2799 1754-2189 |
| DOI: | 10.1038/s41596-020-0392-7 |
| Popis: | The complexity of current nucleic acid isolation methods limits their use outside of the modern laboratory environment. Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick. Nucleic acids can be purified by dipping in-house-made dipsticks into just three solutions: the extract (to bind the nucleic acids), a wash buffer (to remove impurities) and the amplification reaction (to elute the nucleic acids). The speed and simplicity of this method make it ideally suited for molecular applications, both within and outside the laboratory, including limited-resource settings such as remote field sites and teaching institutions. Detailed instructions for how to easily manufacture large numbers of dipsticks in house are provided. Using the instructions, readers can create more than 200 dipsticks in |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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