Prognostic role of ALK-1 and h-TERT expression in glioblastoma multiforme: correlation with ALK gene alterations
| Autor: | Doaa F. Temerik, Marwa T. Hussien, Alia M Attia, A. Hadia, Dalia A. Elsers |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Histology Glioblastoma multiforme Receptor tyrosine kinase Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases medicine Pathology Anaplastic lymphoma kinase RB1-214 Telomerase reverse transcriptase ALK gene gene ALK-1 medicine.diagnostic_test biology business.industry Embryogenesis Retrospective cohort study Prognosis Telomere h-TERT 030104 developmental biology 030220 oncology & carcinogenesis Cancer research biology.protein Immunohistochemistry Original Article business Fluorescence in situ hybridization |
| Zdroj: | Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine, Vol 55, Iss 3, Pp 212-224 (2021) |
| ISSN: | 2383-7837 |
| Popis: | Background Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase that is expressed in the developing central and peripheral nervous systems during embryogenesis. Human telomerase reverse transcriptase (h-TERT) protein resumption is the main process of preservation of telomeres that maintains DNA integrity. The present study aims to evaluate the prognostic role of ALK-1 and h-TERT protein expression and their correlation with ALK gene alterations in glioblastoma multiforme (GBM). Methods The current study is a retrospective study on a cohort of patients with GBM (n = 53) that attempted to detect ALK gene alterations using fluorescence in situ hybridization. ALK-1 and h-TERT proteins were evaluated using immunohistochemistry. Results Score 3 ALK-1 expression was significantly associated with male sex, tumor multiplicity, Ki labeling index (Ki LI), and type of therapeutic modality. Score 3 h-TERT expression exhibited a significant association with Ki LI. ALK gene amplifications (ALK-A) were significantly associated with increased Ki LI and therapeutic modalities. Score 3 ALK-1 protein expression, score 3 h-TERT protein expression, and ALK-A were associated with poor overall survival (OS) and progression-free survival (PFS). Multivariate analysis for OS revealed that ALK gene alterations were an independent prognostic factor for OS and PFS. Conclusions High protein expression of both ALK-1 and h-TERT, as well as ALK-A had a poor impact on the prognosis of GBM. Further studies are needed to establish the underlying mechanisms. |
| Databáze: | OpenAIRE |
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