Use of the mouse aortic ring assay to study angiogenesis
| Autor: | Bernardo Tavora, Gabriela D'Amico, Boris Vojnovic, Marianne Baker, Kairbaan Hodivala-Dilke, Stephen D. Robinson, Tanguy Lechertier, Paul R. Barber, Dylan T. Jones |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Angiogenesis
Neovascularization Physiologic Biology General Biochemistry Genetics and Molecular Biology Neovascularization Tissue Culture Techniques 03 medical and health sciences Mice 0302 clinical medicine In vivo Cell Movement medicine Animals Microscopy Phase-Contrast Microvessel Aorta 030304 developmental biology Cell Proliferation Tube formation 0303 health sciences Cell biology Mice Inbred C57BL Angiopoietin-1 Microscopy Fluorescence 030220 oncology & carcinogenesis Immunology medicine.symptom Immunostaining Ex vivo |
| Zdroj: | Nature protocols. 7(1) |
| ISSN: | 1750-2799 1754-2189 |
| Popis: | Here we provide a protocol for quantitative three-dimensional ex vivo mouse aortic ring angiogenesis assays, in which developing microvessels undergo many key features of angiogenesis over a timescale similar to that observed in vivo. The aortic ring assay allows analysis of cellular proliferation, migration, tube formation, microvessel branching, perivascular recruitment and remodeling-all without the need for cellular dissociation-thus providing a more complete picture of angiogenic processes compared with traditional cell-based assays. Our protocol can be applied to aortic rings from embryonic stage E18 through to adulthood and can incorporate genetic manipulation, treatment with growth factors, drugs or siRNA. This robust assay allows assessment of the salient steps in angiogenesis and quantification of the developing microvessels, and it can be used to identify new modulators of angiogenesis. The assay takes 6-14 d to complete, depending on the age of the mice, treatments applied and whether immunostaining is performed. |
| Databáze: | OpenAIRE |
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