A placebo controlled, dose-ranging, safety study of allogenic mesenchymal stem cells injected by endomyocardial delivery after an acute myocardial infarction
| Autor: | Marian K Rippy, Harold Litt, Seyed M. Hashemi, Susan Ghods, Renu Virmani, Frank D. Kolodgie, Randell G. Young, Kambiz Parcham-Azad, Damir Hamamdzic, Robert L. Wilensky, Martin G. Keane |
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| Rok vydání: | 2007 |
| Předmět: |
Pathology
medicine.medical_specialty Swine Myocardial Infarction Urology Magnetic Resonance Imaging Cine Neovascularization Physiologic Infarction Mesenchymal Stem Cell Transplantation Placebo Placebos Random Allocation Ventricular Dysfunction Left medicine Animals Myocardial infarction Infusions Intralesional Ejection fraction business.industry Myocardium Mesenchymal stem cell medicine.disease Coronary Vessels Disease Models Animal Circulatory system Toxicity Female Stem cell Cardiology and Cardiovascular Medicine business |
| Zdroj: | European Heart Journal. 29:251-259 |
| ISSN: | 1522-9645 0195-668X |
| DOI: | 10.1093/eurheartj/ehm559 |
| Popis: | Aims Although mesenchymal stem cells (MSCs) show promising signs in reducing myocardial infarct (MI) size, the safety of endomyocardial delivery and the most efficacious dose is unknown. Methods and results Three days after MI, female Yorkshire swine (25–32 kg, age 2 months, n = 32) were randomized to endomyocardial delivery of one of three MSC doses (2.4 × 107, 2.4 × 108, 4.4 × 108 cells) or vehicle control. Animals were sacrificed at 12 weeks. There were no safety issues related to cell delivery and all animals tolerated the procedure. By magnetic resonance imaging infarct size (g) was decreased in the experimental groups and increased in the control group; 2.4 × 107: Δ −2.5 ± 2.5 g, 2.4 × 108: −0.9 ± 2.71 g, 4.4 × 108: −1.6 ± 5.8 g, and control +3.6 ± 3.4 g ( P = 0.002, P = 0.016, and P = 0.055 compared with control, respectively). There was no effect on ejection fraction or left ventricular volumes. By histology there were no toxic effects of MSC delivery, however, few engrafted MSCs were observed. Conclusion Direct MSC delivery into infarcted myocardium was safe and produced a local but not a functional effect. There was no dose-dependent effect. The effect of MSCs on infarct reduction may result from transient residence and subsequent paracrine effects. |
| Databáze: | OpenAIRE |
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