Glycolipids and fatty acids of two dog kidney cell lines
| Autor: | C R Gaush, A E Hougland, W R Mayberry |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Magnetic Resonance Spectroscopy
Globotriaosylceramide Biology Kidney Biochemistry Cell Line chemistry.chemical_compound Dogs Glycolipid medicine Animals Molecular Biology chemistry.chemical_classification Ganglioside Cell Membrane Fatty Acids Fatty acid Cell Biology Cell Transformation Viral Kidney cell Membrane medicine.anatomical_structure chemistry lipids (amino acids peptides and proteins) Haematoside Glycolipids Research Article |
| Zdroj: | Biochemical Journal. 177:311-317 |
| ISSN: | 0264-6021 |
| Popis: | Glycolipid and fatty acid compositions were studied in whole cells and plasma membranes from two dog kidney cell lines (Madin-Darby and SV40-transformed cells) grown in monolayer and suspension cultures. Glycolipids, which account for 5% or less of the total lipids in dog kidney cells, were substantially increased in plasma membranes relative to whole cells. Sialoglycolipids more complex than a Tay-Sachs-like ganglioside were not found in any whole-cell or plasma-membrane preparation of this study. Dog kidney cells transformed by SV40 virus contained primarily a less complex sialoglycolipid, haematoside. Neutral glycolipids comprised 26-43% of the total glycolipid content in Madin-Darby preparations, whereas in transformed cells and membranes neutral glycolipids constituted only 1-22% of the total glycolipid content. Ceramide trihexoside was found in Madin-Darby cultures, but not in transformed cultures. The values for short-chain fatty acids from neutral glycolipids and for saturated fatty acids were generally higher than the values for these fatty acids in calf serum. |
| Databáze: | OpenAIRE |
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