Identification and Predictions Regarding the Biosynthesis Pathway of Polyene Macrolides Produced by Streptomyces roseoflavus Men-myco-93-63
| Autor: | Lianna Liu, Jiao Wang, Qingfang Meng, Daqun Liu, Fengying Shen, Xing Li, Yaning Li, Xing Han |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
Antibiotics Polyenes Applied Microbiology and Biotechnology Streptomyces 03 medical and health sciences chemistry.chemical_compound Polyketide Biosynthesis Ascomycota Bacterial Proteins Gene cluster medicine Author Correction 030304 developmental biology Plant Diseases 0303 health sciences Ecology biology Mycelium 030306 microbiology Chemistry fungi biology.organism_classification Antimicrobial Polyene Plant disease Biosynthetic Pathways Biochemistry Biological Control Agents Multigene Family Macrolides Food Science Biotechnology |
| Zdroj: | Appl Environ Microbiol |
| ISSN: | 1098-5336 |
| Popis: | A group of polyene macrolides mainly composed of two constituents was isolated from the fermentation broth of Streptomyces roseoflavus Men-myco-93-63, which was isolated from soil where potato scabs were repressed naturally. One of these macrolides was roflamycoin, which was first reported in 1968, and the other was a novel compound named Men-myco-A, which had one methylene unit more than roflamycoin. Together, they were designated RM. This group of antibiotics exhibited broad-spectrum antifungal activities in vitro against 17 plant-pathogenic fungi, with 50% effective concentrations (EC50) of 2.05 to 7.09 μg/ml and 90% effective concentrations (EC90) of 4.32 to 54.45 μg/ml, which indicates their potential use in plant disease control. Furthermore, their biosynthetic gene cluster was identified, and the associated biosynthetic assembly line was proposed based on a module and domain analysis of polyketide synthases (PKSs), supported by findings from gene inactivation experiments. IMPORTANCEStreptomyces roseoflavus Men-myco-93-63 is a biocontrol strain that has been studied in our laboratory for many years and exhibits a good inhibitory effect in many crop diseases. Therefore, the identification of antimicrobial metabolites is necessary and our main objective. In this work, chemical, bioinformatic, and molecular biological methods were combined to identify the structures and biosynthesis of the active metabolites. This work provides a new alternative agent for the biological control of plant diseases and is helpful for improving both the properties and yield of the antibiotics via genetic engineering. |
| Databáze: | OpenAIRE |
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