Identification of novel transcripts and peptides in developing murine lens
| Autor: | Sean F. Hackett, S. Amer Riazuddin, Firoz Kabir, Muhammad Ali, Mei-Chong Wendy Lee, Ruiqiang Chen, Nader Pourmand, Chan Hyun Na, Shahid Y. Khan |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Proteome Organogenesis lcsh:Medicine Transcriptome Exon Lens Mice Pregnancy Tandem Mass Spectrometry Databases Genetic 2.1 Biological and endogenous factors Aetiology lcsh:Science Pediatric Chromatography Liquid Multidisciplinary High-Throughput Nucleotide Sequencing Exons RNA splicing Female Sequence Analysis Algorithms Biotechnology Sequence analysis Computational biology Biology Article 03 medical and health sciences Databases Genetic Lens Crystalline Genetics Animals Eye Disease and Disorders of Vision Crystalline Sequence Analysis RNA Alternative splicing lcsh:R Intron Exon skipping Introns Alternative Splicing 030104 developmental biology RNA lcsh:Q RNA Splice Sites Peptides Chromatography Liquid |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018) Scientific reports, vol 8, iss 1 Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-28727-w |
| Popis: | We previously investigated the transcriptome and proteome profiles of the murine ocular lens at six developmental time points including two embryonic (E15 and E18) and four postnatal time points (P0, P3, P6, and P9). Here, we extend our analyses to identify novel transcripts and peptides in developing mouse lens. We identified a total of 9,707 novel transcripts and 325 novel fusion genes in developing mouse lens. Additionally, we identified 13,281 novel alternative splicing (AS) events in mouse lens including 6,990 exon skipping (ES), 2,447 alternative 3′ splice site (A3SS), 1,900 alternative 5′ splice site (A5SS), 1,771 mutually exclusive exons (MXE), and 173 intron retention (IR). Finally, we integrated our OMIC (Transcriptome and Proteome) datasets identifying 20 novel peptides in mouse lens. All 20 peptides were validated through matching MS/MS spectra of synthetic peptides. To the best of our knowledge, this is the first report integrating OMIC datasets to identify novel peptides in developing murine lens. |
| Databáze: | OpenAIRE |
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