The biological activity of interferon alpha is influenced by two distinct regions in the protein
| Autor: | Margreet van Heuvel, Kor van As, Ellen C. Zwarthoff, Jan Trapman, Jaap Bosveld, A. C. P. Hekman |
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| Rok vydání: | 1989 |
| Předmět: |
Molecular Sequence Data
Biophysics Alpha interferon Alpha (ethology) Biology medicine.disease_cause Biochemistry Antiviral Agents Cell Line Mice Cricetinae medicine Escherichia coli Animals Amino Acid Sequence Molecular Biology Gene Peptide sequence chemistry.chemical_classification Genetics Biological activity Cell Biology Haplorhini Recombinant Proteins Amino acid Rats chemistry Interferon Type I Homologous recombination Plasmids |
| Zdroj: | Biochemical and biophysical research communications. 164(1) |
| ISSN: | 0006-291X |
| Popis: | With the aim to assign differences in activity between murine interferon-alpha 1 and -alpha 4 to specific amino acids, we have constructed hybrid genes and analysed the antiviral properties of the corresponding hybrid proteins. The hybrid genes were constructed by means of homologous recombination between the alpha 1 and alpha 4 genes in Escherichia coli. Hybrids in which the N-terminal part is derived from alpha 1 show that two regions have a major effect on the activity: amino acid 10-20 and 55-67. When comparing hybrids with N-terminal alpha 4 sequences, transitions in activity are found in the same regions. Interestingly, the curves for the two sets of hybrids are exactly each others mirror image. |
| Databáze: | OpenAIRE |
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