miles-apart-Mediated regulation of cell-fibronectin interaction and myocardial migration in zebrafish
| Autor: | Isao Oishi, Juan Carlos Izpisua Belmonte, Takaaki Matsui, Carles Callol-Massot, Concepción Rodríguez-Esteban, Angel Raya, Yasuhiko Kawakami |
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| Rok vydání: | 2006 |
| Předmět: |
Genotype
Organogenesis Cell Mutant Cell Communication Extracellular matrix Cell Movement Precursor cell medicine Animals Myocytes Cardiac Receptor Zebrafish Genetics biology business.industry General Medicine biology.organism_classification Embryonic stem cell Cell biology Fibronectins Fibronectin Receptors Lysosphingolipid medicine.anatomical_structure Mutation biology.protein Cardiology and Cardiovascular Medicine business |
| Zdroj: | Nature clinical practice. Cardiovascular medicine. 4 |
| ISSN: | 1743-4300 |
| Popis: | The migration of myocardial precursor cells towards the embryonic midline underlies the formation of the heart tube and is a key process of heart organogenesis. The zebrafish mutation miles-apart (mil), which affects the gene encoding a sphingosine-1-phosphate receptor, is characterized by defective migration of myocardial precursor cells and results in the formation of two laterally positioned hearts, a condition known as cardia bifida. The mechanism that disrupts myocardial migration in mil mutants remains largely unclear. To investigate how mil regulates this process, here we analyze the interactions between mil and other mediators of myocardial migration. We show that mil function is associated with the other known cardia bifida locus, natter/fibronectin (nat/fn), which encodes fibronectin, a major component of the extracellular matrix, in the control of myocardial migration. By using a primary culture system of embryonic zebrafish cells, we also show that signaling from the sphingosine-1-phosphate receptor regulates cell–fibronectin interactions in zebrafish. In addition, localized inhibition and activation of cell–fibronectin interactions during the stages of myocardial migration reveal that the temporal regulation of cell–fibronectin interaction by mil is required for proper myocardial migration. Our study reveals novel functional links between sphingosine-1-phosphate receptor signaling and cell–fibronectin interaction in the control of myocardial migration during zebrafish heart organogenesis. |
| Databáze: | OpenAIRE |
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