CsMYB60 directly and indirectly activates structural genes to promote the biosynthesis of flavonols and proanthocyanidins in cucumber
| Autor: | Jialin Li, Jing Han, Zhonghai Ren, Mengyu Liu, Qianqian Luan, Cunjia Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Molecular biology Plant Science Horticulture 01 natural sciences Biochemistry Article 03 medical and health sciences chemistry.chemical_compound Flavonols Biosynthesis Genetics Transcriptional regulation chemistry.chemical_classification biology Binding protein Structural gene food and beverages Promoter biology.organism_classification 030104 developmental biology Flavonoid biosynthesis chemistry Cucumis 010606 plant biology & botany Biotechnology |
| Zdroj: | Horticulture Research |
| ISSN: | 2052-7276 2662-6810 |
| DOI: | 10.1038/s41438-020-0327-z |
| Popis: | Flavonols and proanthocyanidins (PAs) are the main pigments in the black spines of cucumber (Cucumis sativus) fruit, and CsMYB60 is a key regulator of the biosynthesis of flavonols and PAs. However, in cucumber, the tissue distribution pattern of flavonols and PAs and the mechanism of their biosynthesis regulated by CsMYB60 remain unclear. In this study, we clarified the tissue-specific distribution of flavonoids and the unique transcriptional regulation of flavonoid biosynthesis in cucumber. CsMYB60 activated CsFLS and CsLAR by binding to their promoters and directly or indirectly promoted the expression of CsbHLH42, CsMYC1, CsWD40, and CsTATA-box binding protein, resulting in the formation of complexes of these four proteins to increase the expression of Cs4CL and interact with CsTATA-box binding protein to regulate the expression of CsCHS, thereby regulating the biosynthesis of flavonols and PAs in cucumber. Our data provide new insights into the molecular mechanism of flavonoid biosynthesis, which will facilitate molecular breeding to improve fruit quality in cucumber. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|