Labeling and tracking of human pancreatic islets using carbon nanotubes
Autor: | Vittoria Raffa, Marco Bugliani, Valentina Battaglia, Michela Novelli, Franco Filipponi, Matilde Masini, Piero Marchetti, Ugo Boggi, Lorella Marselli, Mara Suleiman, Cristina Riggio, Carlo Bartolozzi, Pellegrino Masiello, Orazio Vittorio, Farooq Syed |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Materials science
Cell Survival Islets of Langerhans Transplantation Biomedical Engineering Contrast Media Pharmaceutical Science Medicine (miscellaneous) Bioengineering Islets of Langerhans Insulin Secretion medicine Animals Humans Insulin Nanotechnology General Materials Science Magnetite Nanoparticles Beta (finance) Incubation Cells Cultured Aged geography geography.geographical_feature_category medicine.diagnostic_test Nanotubes Carbon Pancreatic islets Dextrans Magnetic resonance imaging Anatomy Middle Aged Islet Magnetic Resonance Imaging Molecular biology Rats Transplantation Microscopy Electron medicine.anatomical_structure Microscopy Fluorescence Ultrastructure Beta cell |
Popis: | Limited tools are available for the non-invasive monitoring of transplanted islets. In this study, we have compared the widely used superparamagnetic iron oxide nanoparticle ferumoxide (Endorem) and multiwalled carbon nanotubes (MWCNTs) for islet cell labeling and tracking. INS-1 E cells and human pancreatic islets isolated from 12 non-diabetic cadaveric organ donors (age: 62 ±16 yr, BMI: 24.6 ± 3.3 kg/m2) were incubated with 50 μg/ml Endorem or 15 μg/ml MWCNTs and studied after 7 or 14 days to assess beta cell morphology, ultrastructure, function, cell survival and in-vitro and in-vivo magnetic resonance imaging (MRI). Light and electron (EM) microscopy showed the well-maintained morphology and ultrastructure of both INS-1 E and human islets during the incubation. EM also revealed the presence of Endorem and MWCNTs within the beta but not the alpha cells. The compounds did not affect beta cell function and viability, and in-vitro MRI showed that labeled INS-1 E cells and human islets could be imaged. Finally, MWCNT labeled human islets were successfully transplanted into the subcutis of rats localized in the desired site via magnetic field and tracked by MRI. These data suggest that MWCNTs can be an alternative labeling compound to be used with human islets for experimental and transplantation studies. |
Databáze: | OpenAIRE |
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