Mycobacterium abscessus ESX-3 plays an important role in host inflammatory and pathological responses during infection
| Autor: | Chul-Su Yang, Eun-Kyeong Jo, Mingyu Jung, Myung Hee Kim, Jin Ho Choe, Jichan Jang, Hye-Mi Lee, Jin Kyung Kim, Yi Sak Kim, Loi T. Nguyen, Jin-Man Kim, Soo Yeon Kim, Hyo Sun Jin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Virulence Factors medicine.drug_class medicine.medical_treatment 030106 microbiology Immunology Mutant Mycobacterium abscessus Monoclonal antibody Microbiology Mycobacterium law.invention Pathogenesis 03 medical and health sciences Immune system Bacterial Proteins Antigen law medicine Animals Humans biology Macrophages Macrophage Activation biology.organism_classification Healthy Volunteers Immunity Innate Mice Inbred C57BL 030104 developmental biology Infectious Diseases Cytokine Multigene Family Recombinant DNA Cytokines Female Gene Deletion |
| Zdroj: | Microbes and Infection. 19:5-17 |
| ISSN: | 1286-4579 |
| Popis: | Mycobacterial ESX systems are often related to pathogenesis during infection. However, little is known about the function of ESX systems of Mycobacterium abscessus (Mab). This study focuses on the Mab ESX-3 cluster, which contains major genes such as esxH (Rv0288, low molecular weight protein antigen 7; CFP-7) and esxG (Rv0287, ESAT-6 like protein). An esx-3 (MAB 2224c-2234c)-deletional mutant of Mab (Δesx) was constructed and used to infect murine and human macrophages. We then investigated whether Mab Δesx modulated innate host immune responses in macrophages. Mab Δesx infection resulted in less pathological and inflammatory responses. Additionally, Δesx resulted in significantly decreased activation of inflammatory signaling and cytokine production in macrophages compared to WT. Moreover, recombinant EsxG·EsxH (rEsxGH) proteins encoded by the ESX-3 region showed synergistic enhancement of inflammatory cytokine generation in macrophages infected with Δesx. Taken together, our data suggest that Mab ESX-3 plays an important role in inflammatory and pathological responses during Mab infection. |
| Databáze: | OpenAIRE |
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