Malignant infarction of the middle cerebral artery in a porcine model. A pilot study
| Autor: | Arikan Abelló, Fuat, Martínez-Valverde, Tamara, Sánchez-Guerrero, Ángela, Campos, Mireia, Esteves, Marielle, Gándara, Dario F., Torné, Ramon, Castro, Lidia, Dalmau Bueno, Antoni, Tibau, Joan, Sahuquillo Barris, Juan, Universitat Autònoma de Barcelona |
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| Přispěvatelé: | Producció Animal, Genètica i Millora Animal, Institut Català de la Salut, [Arikan F, Torné R, Sahuquillo J] Servei de Neurocirurgia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Unitat de Recerca en Neurotraumatologia i Neurocirurgia UNINN, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Martínez-Valverde T, Sánchez-Guerrero Á, Castro L] Unitat de Recerca en Neurotraumatologia i Neurocirurgia UNINN, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Campos M, Esteves M] Unitat de Cirurgia Experimental, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Rok vydání: | 2017 |
| Předmět: |
Central Nervous System
0301 basic medicine Microdialysis lcsh:Medicine Infarction Pathology and Laboratory Medicine Vascular Medicine Nervous System 0302 clinical medicine Pig Models Ischemia Animal Cells Occlusion Medicine and Health Sciences Hospital Universitari Vall d’Hebron Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Cerebrovascular Disorders::Brain Ischemia::Brain Infarction::Cerebral Infarction::Infarction Middle Cerebral Artery [DISEASES] lcsh:Science Stroke 61 - Medicina Neurons Cerebral Ischemia Multidisciplinary Penumbra Animal Models Bioassays and Physiological Analysis medicine.anatomical_structure Neurology Experimental Organism Systems Middle cerebral artery Cardiology Anatomy Cellular Types Research Article medicine.medical_specialty enfermedades del sistema nervioso::enfermedades del sistema nervioso central::enfermedades cerebrales::trastornos cerebrovasculares::isquemia cerebral::infarto encefálico::infarto cerebral::infarto de la arteria cerebral media [ENFERMEDADES] Ciències multidisciplinàries Central nervous system Research and Analysis Methods 03 medical and health sciences Signs and Symptoms Diagnostic Medicine Internal medicine medicine.artery medicine cardiovascular diseases business.industry lcsh:R Biology and Life Sciences Cell Biology Infart cerebral Vall d’Hebron Institut de Recerca (VHIR) medicine.disease 030104 developmental biology Cellular Neuroscience lcsh:Q Models animals en la investigació Investigative Techniques::Models Animal::Investigative Techniques::Disease Models Animal [ANALYTICAL DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT] técnicas de investigación::modelos animales::técnicas de investigación::modelos de enfermedad en animales [TÉCNICAS Y EQUIPOS ANALÍTICOS DIAGNÓSTICOS Y TERAPÉUTICOS] business 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona PLoS ONE, Vol 12, Iss 2, p e0172637 (2017) RECERCAT (Dipòsit de la Recerca de Catalunya) Scientia IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Animal models; Central nervous system; Infarction Models animals; Sistema nerviós central; Infart Modelos animales; Sistema nervioso central; Infarto Background and purpose Interspecies variability and poor clinical translation from rodent studies indicate that large gyrencephalic animal stroke models are urgently needed. We present a proof-of-principle study describing an alternative animal model of malignant infarction of the middle cerebral artery (MCA) in the common pig and illustrate some of its potential applications. We report on metabolic patterns, ionic profile, brain partial pressure of oxygen (PtiO2), expression of sulfonylurea receptor 1 (SUR1), and the transient receptor potential melastatin 4 (TRPM4). Methods A 5-hour ischemic infarct of the MCA territory was performed in 5 2.5-to-3-month-old female hybrid pigs (Large White x Landrace) using a frontotemporal approach. The core and penumbra areas were intraoperatively monitored to determine the metabolic and ionic profiles. To determine the infarct volume, 2,3,5-triphenyltetrazolium chloride staining and immunohistochemistry analysis was performed to determine SUR1 and TRPM4 expression. Results PtiO2 monitoring showed an abrupt reduction in values close to 0 mmHg after MCA occlusion in the core area. Hourly cerebral microdialysis showed that the infarcted tissue was characterized by reduced concentrations of glucose (0.03 mM) and pyruvate (0.003 mM) and increases in lactate levels (8.87mM), lactate-pyruvate ratio (4202), glycerol levels (588 μM), and potassium concentration (27.9 mmol/L). Immunohistochemical analysis showed increased expression of SUR1-TRPM4 channels. Conclusions The aim of the present proof-of-principle study was to document the feasibility of a large animal model of malignant MCA infarction by performing transcranial occlusion of the MCA in the common pig, as an alternative to lisencephalic animals. This model may be useful for detailed studies of cerebral ischemia mechanisms and the development of neuroprotective strategies. The Neurotraumatology and Neurosurgery Research Unit is supported by a grant from the Departament d'Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya (SGR 2014-844). This work has been supported in part by the Fondo de Investigación Sanitaria (Instituto de Salud Carlos III) with grant FIS PI11/00700, which was co-financed by the European Regional Development Fund (ERDF) and awarded to Dr. J. Sahuquillo. A. Sánchez-Guerrero is the recipient of a personal pre-doctoral grant from the Instituto de Salud Carlos III (grant number grant number FI12/00074). |
| Databáze: | OpenAIRE |
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