Mycoplasma arthritidis-derived superantigen (MAM) displays DNase activity
| Autor: | Lothar Rink, Silke Overbeck, Maria Lintges, Birgit Plümäkers, Markus Diedershagen, Sabine Arlt |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Immunology Bacterial Toxins Gene Expression chemical and pharmacologic phenomena Biology medicine.disease_cause Mycoplasma arthritidis Microbiology Staphylococcaceae law.invention chemistry.chemical_compound Enterotoxins law hemic and lymphatic diseases medicine Superantigen Escherichia coli Immunology and Allergy Antigens Bacterial Deoxyribonucleases Superantigens Toxin Toxic shock syndrome hemic and immune systems Toxic shock syndrome toxin General Medicine DNA medicine.disease biological factors Recombinant Proteins Infectious Diseases chemistry Recombinant DNA Growth inhibition Deoxyribonuclease I |
| Zdroj: | FEMS immunology and medical microbiology. 49(2) |
| ISSN: | 0928-8244 |
| Popis: | Bacterial superantigens are potent stimulators of the immune system. In this study, we expressed recombinant superantigens, which were then affinity purified and used for growth curves and DNase activity assays. Overexpression of Mycoplasma arthritidis-derived superantigen in Escherichia coli reduced bacterial growth. This is unique, as staphylococcal enterotoxin A and toxic shock syndrome toxin-1, expressed in the same vector system, showed no growth impairment. The observed growth inhibition was caused by the DNase activity of recombinant M. arthritidis-derived superantigen, thus describing the first superantigen showing enzymatic activity, which may be a result of the separate evolution of this toxin. |
| Databáze: | OpenAIRE |
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