Diagnostic rapid tests for acute hantavirus infections: specific tests for Hantaan, Dobrava and Puumala viruses versus a hantavirus combination test
| Autor: | Olli Vapalahti, Ale Närvänen, Pekka Eerikäinen, Ilpo Kuronen, Vesa Koistinen, Helena Hujakka, Antti Vaheri, Åke Lundkvist, Markku Parviainen |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Orthohantavirus Immunodiffusion viruses Hantavirus Infections Cross Reactions Antibodies Viral Puumala virus Serology 03 medical and health sciences Virology Nephropathia epidemica medicine Humans Hantaan virus 030304 developmental biology Hantavirus 0303 health sciences Hantavirus pulmonary syndrome biology 030306 microbiology virus diseases Middle Aged medicine.disease biology.organism_classification 3. Good health Case-Control Studies Hemorrhagic Fever with Renal Syndrome Female Bunyaviridae Hantavirus Infection |
| Zdroj: | Journal of virological methods. 108(1) |
| ISSN: | 0166-0934 |
| Popis: | Hantaviruses infecting humans in Eurasia include Hantaan, Seoul, Puumala and the closely related Dobrava and Saaremaa viruses. These viruses are causative agents of hemorrhagic fever with renal syndrome (HFRS), which is recognized as a severe health care problem in several countries. Diagnostics of hantavirus infections relies on serology, performed principally with enzyme immunoassay (EIA) or immunofluorescence assay (IFA). We developed four 5-min immunochromatographic IgM-antibody tests for diagnostics of acute Puumala, Dobrava and Hantaan virus infections and a similar combination test to detect all Eurasian pathogenic hantavirus infections. We evaluated the assays using 100 fingertip blood samples collected randomly from Finnish volunteers, 28 confirmed hantavirus IgM-negative sera, and 77 sera from patients with acute infections of various hantaviruses. The specificities and sensitivities of the Puumala-, Dobrava- and Hantaan virus -specific tests varied from 96 to 100%, whereas, the combination test showed 96% specificity and 80 to 93% sensitivity. Cross-reactions were observed commonly between the Dobrava and the Hantaan virus tests, but only rarely between the Puumala and the Hantaan virus, or the Puumala and the Dobrava virus, tests. Altogether, the rapid tests showed less cross-reactivity than the respective EIA tests. According to the results, the performance of these tests meets well the requirements for diagnostic use. Nevertheless, the specific one-antigen tests were markedly more sensitive than the combination test. However, if optimized, a combination test would be suitable for regions where several hantaviruses circulate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect