Effect of low- and high-virulence Yersinia enterocolitica strains on the inflammatory response of human umbilical vein endothelial cells
| Autor: | Carine Youta, Martin Ackermann, Geertrui Denecker, Isabelle Lambermont, Sabine Tötemeyer, Guy R. Cornelis, Luís Jaime Mota, Isabelle Stainier, Paul Troisfontaines |
|---|---|
| Přispěvatelé: | UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Cytoplasm
Umbilical Veins Time Factors Immunology Molecular Sequence Data Virulence Down-Regulation Yersinia Microbiology Umbilical vein Type three secretion system Cell Line Mice Bacterial Proteins Animals Humans Interleukin 8 Amino Acid Sequence Serotyping Yersinia enterocolitica Cells Cultured biology Sequence Homology Amino Acid Interleukin-6 Interleukin-8 biology.organism_classification Intercellular Adhesion Molecule-1 Enterobacteriaceae Molecular Pathogenesis Endothelial stem cell Infectious Diseases Hela Cells Parasitology Endothelium Vascular HeLa Cells |
| Zdroj: | Infection and immunity, Vol. 70, no. 7, p. 3510-20 (2002) |
| ISSN: | 7652-7662 |
| Popis: | Pathogenic strains of Yersinia spp. inject a set of Yop effector proteins into eukaryotic cells by using a plasmid-encoded type III secretion system. In this study, we analyzed the inflammatory response of human umbilical vein endothelial cells (HUVECs) after infection with different Yersinia enterocolitica strains. We found that both expression of intercellular adhesion molecule 1 and release of the cytokines interleukin-6 (IL-6) and IL-8 by HUVECs are downregulated in a YopP-dependent way, demonstrating that YopP plays a major role in the inflammatory response of these cells. Infection of HUVECs with several low-virulence (biotype 2, 3, and 4) and high-virulence (biotype 1B) Y. enterocolitica strains showed that biotype 1B isolates are more efficient in inhibiting the inflammatory response than low-virulence Y. enterocolitica strains and that this effect depends on the time of contact. We extended the results of Ruckdeschel et al. and found that on the basis of the presence or absence of arginine-143 of YopP (K. Ruckdeschel, K. Richter, O. Mannel, and J. Heesemann, Infect. Immun. 69: 7652-7662, 2001) all the Y. enterocolitica strains used fell into two groups, which correlate with the low- and high-virulence phenotypes. In addition, we found that high-virulence strains inject more YopP into the cytosol of eukaryotic target cells than do low-virulence strains. |
| Databáze: | OpenAIRE |
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