Cloning and sequencing of the URA3 gene of Kluyveromyces marxianus CBS 6556
| Autor: | J. M. A. Verbakel, Rudi J. Planta, Ruud H. Geerse, Ronald J. M. Bergkamp |
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| Rok vydání: | 1993 |
| Předmět: |
Mutant
Genes Fungal Molecular Sequence Data Orotidine-5'-Phosphate Decarboxylase Bioengineering Molecular cloning Applied Microbiology and Biotechnology Biochemistry Kluyveromyces Transformation Genetic Kluyveromyces marxianus Genetics URA3 Amino Acid Sequence Cloning Molecular Gene Kluyveromyces lactis biology Base Sequence Nucleic acid sequence Chromosome Mapping biology.organism_classification Molecular biology genomic DNA Mutation Chromosomes Fungal Biotechnology |
| Zdroj: | Yeast (Chichester, England). 9(6) |
| ISSN: | 0749-503X |
| Popis: | The URA3 gene, coding for orotidine-5′-phosphate decarboxylase, from Kluyveromyces marxianus CBS 6556, was isolated from a genomic DNA library. The K. marxianus URA3 gene encodes a protein of 267 amino acids with a calculated molecular weight of 29·3 kDa. Comparison of the K. marxianus protein with the corresponding enzymes of Saccharomyces cerevisiae and Kluyveromyces lactis showed amino acid sequence identifies of 81% and 88%, respectively. Using contour-clamped homogeneous electric field gel electrophoresis, the genomic copy was found to be located on chromosome VI. We have used the cloned gene for the construction of a K. marxianus leu2 mutant. This mutant contains no heterologous sequences, which is essential to make it acceptable for application in the food industry. |
| Databáze: | OpenAIRE |
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