Polysaccharide-layered double hydroxide–aldolase biohybrid beads for biocatalysed CC bond formation
| Autor: | Vanessa Prevot, Christine Guérard-Hélaine, Claude Forano, Céline Laroche, Philippe Michaud, Rima Mahdi, Marielle Lemaire |
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| Přispěvatelé: | Institut de Chimie de Clermont-Ferrand (ICCF), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-SIGMA Clermont (SIGMA Clermont)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Pascal (IP), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
Fructose-bisphosphate aldolase
Bioengineering engineering.material Polysaccharide Biochemistry Catalysis Nanomaterials chemistry.chemical_compound Aldolase Bioreactor [CHIM]Chemical Sciences Organic chemistry chemistry.chemical_classification biology Process Chemistry and Technology Aldolase A Layered double hydroxides Nanomaterial Biohybrid ι-Carrageenan chemistry Chemical engineering Layered double hydroxide engineering biology.protein Hydroxide |
| Zdroj: | Journal of Molecular Catalysis B: Enzymatic Journal of Molecular Catalysis B: Enzymatic, Elsevier, 2015, 122, pp.204-211. ⟨10.1016/j.molcatb.2015.07.014⟩ Journal of Molecular Catalysis B: Enzymatic, 2015, 122, pp.204-211. ⟨10.1016/j.molcatb.2015.07.014⟩ |
| ISSN: | 1381-1177 |
| DOI: | 10.1016/j.molcatb.2015.07.014 |
| Popis: | International audience; This study describes the design a novel microbioreactor for the biocatalysed asymmetric synthesis of polyhydroxylated compounds. The bioreactor was elaborated by a hierarchical assembly of a recombinant fructose-6-phosphate aldolase (FSAwt), layered double hydroxides (LDH) nanoplatelets and polysaccharide beads. Various polysaccharides were tested to encapsulate FSAwt and pre-immobilised FSAwt in LDH (FSAwt@Mg2Al-LDH). ι-Carrageenan appears as the most suitable candidate to prepare biohybrid beads. The retained activities in ι-carrageenan beads were measured for both free FSAwt and immobilised FSAwt revealing a 3 fold higher activity in favour of the pre-immobilised FSA. This result was probably due to a positive interaction between the negatively charged polysaccharide and the positively charged LDH layers. The catalytic activity, the recyclability and the storage of the beads were studied in addition to the leakage of FSAwt activity from beads. Nanostructuration of both FSAwt/ι-carr and FSAwt@Mg2Al-LDH/ι-carr was investigated by structural and textural characterization techniques. |
| Databáze: | OpenAIRE |
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