Expression of Nitric Oxide Synthase and Nitric Oxide Levels in Peripheral Blood Cells and Oxidized Low-Density Lipoprotein Levels in Saliva as Early Markers of Severe Dengue

Autor: Nimanthi Jayathilaka, K.N. Seneviratne, Ranjan Premaratna, Suharshi S. Perera, Harsha Hapugaswatta, Ruwani L. Wimalasekara
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Adult
Male
medicine.medical_specialty
Saliva
Adolescent
Article Subject
Nitric Oxide Synthase Type II
Nitric Oxide
medicine.disease_cause
Gastroenterology
Sensitivity and Specificity
General Biochemistry
Genetics and Molecular Biology
Severe dengue
Dengue fever
Nitric oxide
03 medical and health sciences
Young Adult
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
medicine
Humans
Severe Dengue
Retrospective Studies
General Immunology and Microbiology
biology
business.industry
General Medicine
Middle Aged
medicine.disease
Peripheral blood
Lipoproteins
LDL
Nitric oxide synthase
030104 developmental biology
Endocrinology
chemistry
030220 oncology & carcinogenesis
Case-Control Studies
biology.protein
Medicine
Female
business
Biomarkers
Oxidative stress
Research Article
Lipoprotein
Zdroj: BioMed Research International, Vol 2021 (2021)
BioMed Research International
ISSN: 2314-6133
DOI: 10.1155/2021/6650596
Popis: Background. Severe dengue (SD), experienced by only a fraction of dengue patients, can be lethal. Due to the lack of early markers that can predict the evolution of SD, all dengue patients have to be monitored under hospital care. We discovered early oxidative stress markers of SD to identify patients who can benefit from early intervention before the symptoms appear. Methods. The expression of inducible nitric oxide synthase (iNOS) in peripheral blood cells (PBC), nitric oxide (NO), and oxidized low-density lipoprotein (oxLDL) levels in plasma and saliva collected at early stages of dengue infection from 20 nonsevere dengue fever (DF) patients and 20 patients who later developed SD were analyzed in a retrospective nested case-control study. Results. The expression of iNOS is significantly ( P < 0.05 ) lower in patients who developed SD than in DF patients at admission within 4 days from fever onset. Median plasma NO concentration within 4 days from fever onset is also significantly ( P < 0.05 ) lower in patients who developed SD ( 17.9 ± 1.6 μ mol / L ) than DF ( 23.0 ± 2.1 μ mol / L ). Median oxLDL levels in plasma within 3 days from fever onset is significantly ( P < 0.05 ) lower in patients who developed SD ( 509.4 ± 224.1 ng / mL ) than DF ( 740.0 ± 300.0 ng / mL ). Median salivary oxLDL levels are also significantly ( P < 0.05 ) lower in patients who developed SD ( 0.8 ± 0.5 ng / mL ) than DF ( 3.6 ± 2.6 ng / mL ) within 4 days from fever onset. Conclusions. These findings suggest that the expression of iNOS (73% sensitivity, 86% specificity) and plasma NO (96% sensitivity, 61% specificity at 22.3 μmol/L; P < 0.05 ) may serve as early markers of SD within 3 days from fever onset. Salivary oxLDL levels may serve as early noninvasive markers of SD with a sensitivity and specificity, respectively, of 57% and 91% at 0.9 ng/mL; 76% and 55% at 2.3 ng/mL; and 100% and 50% at 4.6 ng/mL ( P < 0.05 ) within 4 days from fever onset.
Databáze: OpenAIRE
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