Human and Monkey Corneal Endothelium Expression of Low-Density Lipoprotein Receptors
| Autor: | Mark A. Pavilack, Susan G. Elner, Beatrice Y.J.T. Yue, Harry R. Davis, Victor M. Elner, Joseph A. Cornicelli |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Corneal endothelium
Endothelium Cell Survival Cell Biology Acetoacetates Cornea Extracellular matrix chemistry.chemical_compound medicine Animals Humans Receptor Cells Cultured Fluorescent Dyes Wound Healing Endothelium Corneal Carbocyanines Macaca mulatta Cell biology Lipoproteins LDL Ophthalmology medicine.anatomical_structure Receptors LDL chemistry Biochemistry Cell culture Child Preschool Low-density lipoprotein lipids (amino acids peptides and proteins) Rabbits Cell Division Corneal Injuries Lipoprotein |
| Zdroj: | American Journal of Ophthalmology. 111:84-91 |
| ISSN: | 0002-9394 |
| DOI: | 10.1016/s0002-9394(14)76902-8 |
| Popis: | Receptors for low-density lipoprotein are necessary for high-affinity uptake of lipid and protein essential to cell structure and function. Distinct receptors for acetoacetylated low-density lipoprotein internalize oxidized or enzymatically modified low-density lipoprotein and extracellular matrix components. We identified low-density lipoprotein receptors on cultured human and monkey corneal endothelial cells by the avid incorporation of fluorescently labeled low-density lipoprotein that was competitively inhibited by excess unlabeled low-density lipoprotein but not by unlabeled acetoacetylated low-density lipoprotein. Specific uptake of labeled low-density lipoprotein was greatest in nonconfluent, growing cells and increased after low-density lipoprotein deprivation. Intact endothelial monolayers of whole human corneas also incorporated low-density lipoprotein but not acetoacetylated low-density lipoprotein. After scratch injury of human corneas, spreading endothelium adjacent to areas of cell loss internalized more fluorescent low-density lipoprotein than cells distant from the injury. Blood-aqueous barrier breakdown occurring in ocular diseases and after surgical and nonsurgical trauma may allow leakage of circulating low-density lipoprotein, which provides a rich supply of lipid and protein for endothelial use. Efficient, receptor-mediated, low-density lipoprotein uptake may facilitate repair of damaged corneal endothelial membranes and regeneration of intact, functional cell monolayers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|