Influence of the duration of ischemia and reperfusion on infarct volume and microvascular damage in mice
| Autor: | Moritz Gröger, Gabriele Jäger, Dorothe Burggraf, Gerhard F. Hamann, Milan R. Vosko, Martin Liebetrau, Nathalie Wunderlich, Nikolaus Plesnila |
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| Rok vydání: | 2006 |
| Předmět: |
Brain Infarction
Collagen Type IV Male medicine.medical_specialty Middle Cerebral Artery Ischemia Infarction Vascular permeability Basement Membrane Brain Ischemia Mice Western blot Internal medicine Medicine Animals cardiovascular diseases Collagen type medicine.diagnostic_test business.industry Microcirculation Brain Endothelial Cells Infarction Middle Cerebral Artery General Medicine medicine.disease Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Neurology Anesthesia Reperfusion Injury Infarct volume Cardiology Immunohistochemistry Basal lamina Neurology (clinical) business |
| Zdroj: | Neurological research. 28(2) |
| ISSN: | 0161-6412 |
| Popis: | Focal cerebral ischemia is responsible for alterations of vascular permeability, and the loss of microvascular integrity is a primary source of subsequent hemorrhages. We evaluated the influence of different durations of ischemia and reperfusion on infarction size and microvascular damage after focal cerebral ischemia in the mouse.C57BL/6 mice (n=39) were subjected to focal cerebral ischemia (I) and reperfusion (R). Consecutive brain sections were analysed for infarction volumes (Nissl-staining) and for collagen type IV (immunohistochemistry and western blot).Infarction size (percentage of the infarction volume versus ipsilateral hemisphere) increased with total time of ischemia and reperfusion: 19+/-2% (I3R0), 30+/-2% (I3R3), 36+/-4% (I3R12), 41+/-4% (I1R24), 45+/-6% (I2R24) and 58+/-2% (I3R24). The ischemic hemispheres showed a significant progressive reduction of collagen type IV positive vessels (ischemic versus non-ischemic contralateral area): 90+/-3% (I3R0), 88+/-1% (I3R3), 82+/-3% (I3R12), 85+/-3% (I1R24), 79+/-3% (I2R24), 72+/-2% (I3R24).Both prolonged ischemia and reperfusion lead to an increased infarction volume, as well as progressive microvascular damage. |
| Databáze: | OpenAIRE |
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