An amino-terminal fragment of LCRF, LCRF-(1-35), has the same activity as the natural peptide
| Autor: | Gary M. Green, Difu Guan, Alan W. Spannagel, J. R. Reeve, Rodger A. Liddle |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Duodenum Physiology Peptide Biology Devazepide Sincalide Gastrointestinal Hormones Hormone Antagonists Pancreatic Juice Physiology (medical) Animals Secretion Rats Wistar Growth Substances Pancreas Peptide sequence Cholecystokinin chemistry.chemical_classification Benzodiazepinones Hepatology Gastroenterology Biological activity Peptide Fragments Rats Amino acid Molecular Weight Gastrointestinal hormone chemistry Biochemistry Trypsin Inhibitor Kazal Pancreatic Intercellular Signaling Peptides and Proteins Gastrointestinal function |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 273:G754-G758 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.1997.273.3.g754 |
| Popis: | A cholecystokinin (CCK)-releasing peptide, luminal CCK-releasing factor (LCRF), has been purified from rat jejunal secretion. Amino acid analysis and mass spectral analysis showed that the purified peptide is composed of 70-75 amino acid residues and has a mass of 8,136 Da. Microsequence analysis of LCRF yielded an amino acid sequence for the amino-terminal 41 residues. To determine the biologically active region of the molecule, a peptide was synthesized consisting of the amino-terminal 35 amino acids of LCRF. In this study, intraduodenal infusion of LCRF-(1-35) significantly stimulated pancreatic secretion in conscious rats. The dose-response curves to LCRF-(1-35) and to monitor peptide were similar and biphasic, with higher doses producing submaximal pancreatic secretory responses. The CCK-A receptor antagonist MK-329 abolished the pancreatic secretory response to intraduodenally infused LCRF-(1-35). These results demonstrate that LCRF biological activity is contained within the amino-terminal 35-amino acid portion of LCRF, and this fragment may be useful for investigating the role of LCRF in gastrointestinal function. |
| Databáze: | OpenAIRE |
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