Analysis of the promoter region of the human FcRn gene
| Autor: | Mikulska J, Neil E. Simister |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Regulation of gene expression
Reporter gene 5' flanking region Histocompatibility Antigens Class I Biophysics Promoter Receptors Fc Biology Biochemistry Molecular biology Chloramphenicol acetyltransferase AP-1 transcription factor Gene Expression Regulation Structural Biology Genes Reporter Genetics Humans Promoter Regions Genetic Gene Transcription factor Transcription Factors |
| Zdroj: | Biochimica et biophysica acta. 1492(1) |
| ISSN: | 0006-3002 |
| Popis: | The 5′-flanking region of the human FcRn α-chain gene was analyzed for its ability to directly express the chloramphenicol acetyltransferase (CAT) reporter gene in NIH3T3 and Lu106 cells. Transient transfection of the CAT constructs revealed that there was promoter activity in the region −660 to +300 of the 5′-flanking sequence. Electrophoretic mobility-shift assays showed that there are functional binding sites for Sp1 or Sp1-like factors, AP1 or a related factor, and additional unidentified proteins in the promoter region. |
| Databáze: | OpenAIRE |
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