Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis
| Autor: | Alejandro Ruiz-Argüelles, Hilda Salgado-Rosas, Abdirashid Shire, Ricardo Pérez-Fuentes, John P. Ackers, Patricia Talamás-Rohana, María Del Carmen Sánchez-Guillén |
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| Rok vydání: | 2002 |
| Předmět: |
Cellular immunity
Time Factors T-Lymphocytes Dispar CD4-CD8 Ratio Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Microbiology Entamoeba Interferon-gamma Entamoeba histolytica fluids and secretions Virology parasitic diseases medicine Animals Humans Amoebiasis Mexico Immunity Cellular Entamoebiasis biology biology.organism_classification medicine.disease Infectious Diseases Acute Disease Antibody Formation Chronic Disease Humoral immunity Immunology Dysentery Amebic biology.protein Cytokines Parasitology Interleukin-4 Antibody Asymptomatic carrier |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 66:731-737 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.2002.66.731 |
| Popis: | To correlate a particular state of immunity with Entamoeba spp., we used colorimetric PCR to differentiate E. histolytica from E. dispar in individuals with amoebiasis and to associate its presence with the clinical profile, including humoral and cellular immune responses to E. histolytica. Our results showed high levels of antibody in acute amoebiasis and elevation of IL-4 production, a cytokine related to Th2 profile, associated with E. histolytica. In chronic amoebiasis, even with anti-E. histolytica seropositivity, intestinal symptoms were associated with E. dispar in all the cases, without differences in level of antibodies, BTI, CD4+/CD8+ ratio, INF-gamma, and IL-4. Among asymptomatic carriers, E. dispar was more frequently found; however, identification of E. histolytica in two asymptomatic carriers associated with high levels of INF-gamma, a cytokine related to Th1 profile, demonstrate the importance of making specific diagnosis of Entamoeba spp., to establish the clinical and epidemiological behavior in both intestinal and extra-intestinal amoebiasis. |
| Databáze: | OpenAIRE |
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