Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men
| Autor: | Miroslava Janković, Jelena Danilović Luković, Ninoslav Mitić, Tamara Janković, Sanja Goč |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Glycan Wheat Germ Agglutinins lcsh:Medicine 030209 endocrinology & metabolism Context (language use) surface glycans seminal prostasomes Chromatography Affinity lectin-affinity chromatography Extracellular Vesicles 03 medical and health sciences 0302 clinical medicine Semen Concanavalin A Humans Medicine Transferase chemistry.chemical_classification 030219 obstetrics & reproductive medicine biology business.industry Cell Membrane lcsh:R Prostate Oligospermia gamma-Glutamyltransferase Articles General Medicine Wheat germ agglutinin Biochemistry chemistry Case-Control Studies biology.protein Alkaline phosphatase Prostasomes gamma-glutamyl transferase business Glycoprotein alkaline phosphatase |
| Zdroj: | Upsala Journal of Medical Sciences, Vol 125, Iss 1, Pp 10-18 (2020) Upsala Journal of Medical Sciences |
| ISSN: | 2000-1967 0300-9734 |
| DOI: | 10.1080/03009734.2019.1690603 |
| Popis: | Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs) whose composition is, additionally, influenced by different physiological conditions. Aiming at the molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation. Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference could upgrade standardization of EVs isolated by different methods and intended for use in biomedicine. Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic (sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns. Prostasomes recovered in LAC-separated fractions were characterized according to the distribution of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin CD63, and total protein/glycoprotein composition. Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing in GGT activity and surface glycans were separated on the WGA column. Additional populations having distinct profiles of total glycoproteins and which can be tracked down by ALP activity were enriched on the Con A column. WGA-separated populations were similar in sPro-N and sPro-O, whereas Con A-separated ones were strikingly different. Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic and oligozoospermic men. |
| Databáze: | OpenAIRE |
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