Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitro
| Autor: | Gyu Li Kim, Hidetaka Nagata, Rena Kono, Ryuta Koyama, Yuji Ikegaya |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Amyloid beta
hippocampus Immunocytochemistry Hippocampus Micro Reports Hippocampal formation Postsynapse Presynapse neuronal activity Synapse Micro Report Mice synapse Alzheimer Disease Premovement neuronal activity Animals Pharmacology (medical) Cells Cultured Pharmacology Neurons Amyloid beta-Peptides biology Chemistry Alzheimer's disease Peptide Fragments amyloid beta Psychiatry and Mental health Clinical Psychology nervous system Synapses biology.protein Neuroscience |
| Zdroj: | Neuropsychopharmacology Reports |
| ISSN: | 2574-173X |
| Popis: | Aim The accumulation of amyloid beta (Aβ) is one of the characteristics of Alzheimer's disease. The excessive accumulation of Aβ has been suggested to result in a decrease in the number of synapses. Although the number of synapses is generally modulated by neuronal activity, whether neuronal activity affects Aβ‐induced synapse loss remains unknown. Therefore, we addressed this question using a primary culture of hippocampal neurons. Method The neuronal activity of cultured hippocampal neurons from mouse pups was increased using the chemogenetic technique designer receptors exclusively activated by designer drugs (DREADD). The cultured neurons were treated with Aβ, and synapse density was assessed by immunocytochemistry. Results Aβ decreased the synapse density probably by decreasing postsynapse. On the other hand, enhanced neuronal activity did not affect the synapse density significantly. However, there was a trend that enhanced neuronal activity increased especially presynapse density. Conclusion We found that enhanced neuronal activity did not affect Aβ‐induced synapse loss in vitro. We investigated whether DREADD system‐mediated enhancement of neuronal activity modulates Aβ‐induced synaptic loss in the primary culture of hippocampal neurons, finding that enhanced neuronal activity does not attenuate Aβ‐induced synaptic loss. |
| Databáze: | OpenAIRE |
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