Lagerstroemia Speciosa (L.) Pers Leaf Extract Attenuates Lung Tumorigenesis via Alleviating Oxidative Stress, Inflammation and Apoptosis

Autor:	Sherien K. Hassan, Nermin M. El-Sammad, Wagdy K. B. Khalil, Mahmoud A.M. Nawwar, Amria M. Mousa, Elsayed A. Elsayed, A N Hashim, Abeer H. Abdel-Halim, Nayera Anwar
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0301 basic medicine Antioxidant medicine.medical_treatment Pharmacology medicine.disease_cause Biochemistry Article Lagerstroemia speciosa Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine oxidative stress Lung cancer Molecular Biology A549 cell Chemistry apoptosis respiratory system medicine.disease respiratory tract diseases lung cancer 030104 developmental biology Benzo(a)pyrene Apoptosis inflammation 030220 oncology & carcinogenesis benzo(a)pyrene Carcinogenesis Oxidative stress
Zdroj:	Biomolecules Volume 9 Issue 12
ISSN:	2218-273X
Popis:	One of the major etiological factors that account for lung cancer is tobacco use. Benzo(a)pyrene [B(a)P], one of the main constituents of tobacco smoke, has a key role in lung carcinogenesis. The present study was conducted to investigate the cytotoxicity of an aqueous ethanolic extract of Lagerstroemia speciosa (L.) Pers leaves (LLE) on human lung adenocarcinoma cells (A549), as well as its in vivo antitumor effect on a lung tumorigenesis mice model. Our results revealed that LLE possesses cytotoxic activity against the A549 cell line. Mice orally administered B(a)P (50 mg/kg body weight) showed an increase in relative lung weight with subsequent decrease in final body weight. Serum levels of tumor marker enzymes AHH, ADA and LDH and the inflammatory mediator NF-&kappa B increased, while total antioxidant capacity (TAC) decreased. In addition, we observed the increased activity of metalloproteinases (MMP-2 and MMP-12) and levels of the tumor angiogenesis marker VEFG and the lipid peroxidation marker MDA, as well as decreased levels of the non-enzymatic antioxidant GSH and enzymatic antioxidants CAT and GSH-Px in lung tissues. Moreover, B(a)P administration up-regulated the expression of the COX-2 gene, pro-inflammatory cytokines TNF-&alpha and IL-6, and an anti-apoptotic gene Bcl-2, and at the same time down-regulated expression of pro-apoptotic genes BAX and caspase-3 and the p53 gene. Pre- and post-treatment with LLE (250 mg/kg body weight) attenuated all these abnormalities. Histopathological observations verified the protective effect of LLE. Overall, the present data positively confirm the potent antitumor effect of L. speciosa leaves against lung tumorigenesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a1c63c0cd4d1c4a65275f720f71d2a0 http://europepmc.org/articles/PMC6995620 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.