Portable platform for rapid in-field identification of human fecal pollution in water
| Autor: | Sanchita Bhadra, Yu Sherry Jiang, Andrew D. Ellington, Jessica A. Popoola, Barrett R. Morrow, Sheng Cai, Timothy E. Riedel |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pollution Genetic Markers Environmental Engineering media_common.quotation_subject 030106 microbiology Loop-mediated isothermal amplification Computational biology Article 03 medical and health sciences Feces Nucleic Acids Environmental monitoring False positive paradox Bacteroides Humans Waste Management and Disposal Water Science and Technology Civil and Structural Engineering media_common Sewage Ecological Modeling Water Pollution Amplicon Fecal coliform Ecological Modelling Identification (information) Environmental science Water Microbiology Environmental Monitoring |
| Zdroj: | Water Research |
| ISSN: | 1879-2448 |
| Popis: | Human fecal contamination of water is a public health risk. However, inadequate testing solutions frustrate timely, actionable monitoring. Bacterial culture-based methods are simple but typically cannot distinguish fecal host source. PCR assays can identify host sources but require expertise and infrastructure. To bridge this gap we have developed a field-ready nucleic acid diagnostic platform and rapid sample preparation methods that enable on-site identification of human fecal contamination within 80 min of sampling. Our platform relies on loop-mediated isothermal amplification (LAMP) of human-associated Bacteroides HF183 genetic markers from crude samples. Oligonucleotide strand exchange (OSD) probes reduce false positives by sequence specifically transducing LAMP amplicons into visible fluorescence that can be photographed by unmodified smartphones. Our assay can detect as few as 17 copies/ml of human-associated HF183 targets in sewage-contaminated water without cross-reaction with canine or feline feces. It performs robustly with a variety of environmental water sources and with raw sewage. We have also developed lyophilized assays and inexpensive 3D-printed devices to minimize cost and facilitate field application. |
| Databáze: | OpenAIRE |
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