Characterization of influenza A viruses isolated from wild waterfowl in Zambia
| Autor: | Bernard M. Hang’ombe, Wilbroad Chansa, Jack Chulu, Chuma Simukonda, Boniface Namangala, Yuka Suzuki, Manabu Igarashi, Chihiro Sugimoto, Ayato Takada, Hiroshi Kida, Ichiro Nakamura, Aaron S. Mweene, Akihiro Ishii, Kimihito Ito, Rashid Manzoor, Hirofumi Sawa, Edgar Simulundu, Ladslav Moonga |
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| Rok vydání: | 2011 |
| Předmět: |
animal diseases
Molecular Sequence Data Orthomyxoviridae Zambia Animals Wild medicine.disease_cause Virus Gene flow Feces Mice Viral Proteins Anseriformes Virology Influenza A virus medicine Waterfowl Animals Humans Phylogeny Mice Inbred BALB C Virulence biology Phylogenetic tree virus diseases biology.organism_classification Influenza A virus subtype H5N1 Viral replication Influenza in Birds |
| Zdroj: | Journal of General Virology. 92:1416-1427 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Although the quest to clarify the role of wild birds in the spread of the highly pathogenic H5N1 avian influenza virus (AIV) has yielded considerable data on AIVs in wild birds worldwide, information regarding the ecology and epidemiology of AIVs in African wild birds is still very limited. During AIV surveillance in Zambia (2008–2009), 12 viruses of distinct subtypes (H3N8, H4N6, H6N2, H9N1 and H11N9) were isolated from wild waterfowl. Phylogenetic analyses demonstrated that all the isolates were of the Eurasian lineage. Whilst some genes were closely related to those of AIVs isolated from wild and domestic birds in South Africa, intimating possible AIV exchange between wild birds and poultry in southern Africa, some gene segments were closely related to those of AIVs isolated in Europe and Asia, thus confirming the inter-regional AIV gene flow among these continents. Analysis of the deduced amino acid sequences of internal proteins revealed that several isolates harboured particular residues predominantly observed in human influenza viruses. Interestingly, the isolates with human-associated residues exhibited higher levels of virus replication in the lungs of infected mice and caused more morbidity as measured by weight loss than an isolate lacking such residues. This study stresses the need for continued monitoring of AIVs in wild and domestic birds in southern Africa to gain a better understanding of the emergence of strains with the potential to infect mammals. |
| Databáze: | OpenAIRE |
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