Tek/Tie2 is not required for cardiovascular development in zebrafish
| Autor: | Ingo Ebersberger, Didier Y.R. Stainier, Srinivas Allanki, Claudia Carlantoni, Zhen Jiang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
animal structures
Morpholino Organogenesis Mutant Locus (genetics) Cardiovascular System Angiopoietin 03 medical and health sciences 0302 clinical medicine Animals Allele Molecular Biology Zebrafish Phylogeny 030304 developmental biology Gene Editing 0303 health sciences biology Embryo Zebrafish Proteins biology.organism_classification Angiopoietin receptor Receptor TIE-2 Cell biology embryonic structures biology.protein 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.193029 |
| Popis: | Angiopoietin/TIE signalling plays a major role in blood and lymphatic vessel development. In mouse, Tek/Tie2 mutants die prenatally due to a severely underdeveloped cardiovascular system. In contrast, in zebrafish, previous studies have reported that while embryos injected with tek morpholinos (MOs) exhibit severe vascular defects, tek mutants display no obvious vascular malformations. To further investigate the function of zebrafish Tek, we generated a panel of loss-of-function tek mutants, including RNA-less alleles, an allele lacking the MO-binding site, an in-frame deletion allele, and a premature termination codon-containing allele. Our data show that all these mutants survive to adulthood with no obvious cardiovascular defects. MO injections into tek mutants lacking the MO-binding site or the entire tek locus cause similar vascular defects as those observed in MO-injected +/+ siblings, indicating off-target effects of the MOs. Surprisingly, comprehensive phylogenetic profiling and synteny analyses reveal that Tek was lost in the largest teleost clade, suggesting a lineage-specific shift in the function of TEK during vertebrate evolution. Altogether, these data show that Tek is dispensable for zebrafish development, and probably dispensable in most teleost species. |
| Databáze: | OpenAIRE |
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