Restraint stress increased the permeability of the nasal epithelium in BALB/c mice
Autor: | Víctor Rivera-Aguilar, Antonio Franco-Vadillo, Luz María Cárdenas-Jaramillo, Mireille Toledo-Blas, Juan Manuel Gutiérrez-Meza, Rosa Adriana Jarillo-Luna |
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Rok vydání: | 2020 |
Předmět: |
Restraint
Physical Nasal cavity medicine.medical_specialty Endocrinology Diabetes and Metabolism Mucous membrane of nose Occludin Tight Junctions Mice 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine medicine Animals Claudin Biological Psychiatry Mice Inbred BALB C Tight junction Endocrine and Autonomic Systems Chemistry Dextrans 030227 psychiatry Nasal Mucosa Psychiatry and Mental health medicine.anatomical_structure Membrane protein Paracellular transport Corticosterone Fluorescein-5-isothiocyanate Stress Psychological 030217 neurology & neurosurgery Respiratory tract |
Zdroj: | Psychoneuroendocrinology. 117:104700 |
ISSN: | 0306-4530 |
Popis: | Stress seems to affect the onset and evolution of diverse illnesses with an inflammatory substrate. Whether physiological or psychological, stress increases epithelial permeability. In the mucosa of the nasal cavity and upper respiratory tract, the epithelial barrier is regulated in large part by bicellular and tricellular tight junctions (bTJs and tTJs, respectively). The junctional complexes are composed of multiple membrane proteins: claudins, tight-junction-associated MARVEL proteins (TAMs: occludin, tricellulin and marvelD3), and scaffolding proteins such as ZO-1, -2 and -3. The aim of the present study was to examine the possible modification of nasal permeability and TJ protein expression in a mouse model of acute psychological stress (a 4-h immobility session). Serum corticosterone was quantified from plasma samples to verify the onset of stress. Evaluation was made of the relative concentration of key proteins in nasal mucosa by using Western blot, and of changes in permeability by analyzing FITC-Dextran leakage from the nose to the blood. Compared to the control, the stressed group showed a greater epithelial permeability to FITC-Dextran, a reduced expression of occludin and tricellulin, and an elevated expression of ZO-2 and claudin-4. This evidence points to increased paracellular flow of large molecules through an altered structure of tTJs. Apparently, the structure of bTJs remained unchanged. The current findings could provide insights into the relation of stress to the onset/exacerbation of respiratory infections and/or allergies. |
Databáze: | OpenAIRE |
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