Co-infection by alveolate parasites and frog virus 3-like ranavirus during an amphibian larval mortality event in Florida, USA
| Autor: | Maki Tabuchi, Jan H. Landsberg, Yasunari Kiryu, Thomas B. Waltzek, Asa Preston, Sarah Reintjes-Tolen, Kevin M. Enge, Allan P. Pessier |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Diseases of Aquatic Organisms. 105:89-99 |
| ISSN: | 1616-1580 0177-5103 |
| Popis: | A multispecies amphibian larval mortality event, primarily affecting American bull- frogs Lithobates catesbeianus, was investigated during April 2011 at the Mike Roess Gold Head Branch State Park, Clay County, Florida, USA. Freshly dead and moribund tadpoles had hemor- rhagic lesions around the vent and on the ventral body surface, with some exhibiting a swollen abdomen. Bullfrogs (100%), southern leopard frogs L. sphenocephalus (33.3%), and gopher frogs L. capito (100%) were infected by alveolate parasites. The intensity of infection in bullfrog livers was high. Tadpoles were evaluated for frog virus 3 (FV3) by histology and PCR. For those southern leopard frog tadpoles (n = 2) whose livers had not been obscured by alveolate spore infection, nei- ther a pathologic response nor intracytoplasmic inclusions typically associated with clinical infec- tions of FV3-like ranavirus were noted. Sequencing of a portion (496 bp) of the viral major capsid protein gene confirmed FV3-like virus in bullfrogs (n = 1, plus n = 6 pooled) and southern leopard frogs (n = 1, plus n = 4 pooled). In July 2011, young-of-the-year bullfrog tadpoles (n = 7) were neg- ative for alveolate parasites, but 1 gopher frog tadpole was positive. To our knowledge, this is the first confirmed mortality event for amphibians in Florida associated with FV3-like virus, but the extent to which the virus played a primary role is uncertain. Larval mortality was most likely caused by a combination of alveolate parasite infections, FV3-like ranavirus, and undetermined etiological factors. |
| Databáze: | OpenAIRE |
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