Acetylcholinesterase Inhibition Promotes Retinal Vasoprotection and Increases Ocular Blood Flow in Experimental Glaucoma
| Autor: | Adriana Di Polo, Jessica N. MacIntyre, Christian Casanova, Mohammadali Almasieh, Mylene Pouliot, Elvire Vaucher, Melanie E. M. Kelly |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Retinal Ganglion Cells Intraocular pressure medicine.medical_specialty genetic structures Cell Survival medicine.drug_class Glaucoma Ocular hypertension Cell Count chemistry.chemical_compound Rats Inbred BN Ophthalmology medicine Animals Fluorescent Antibody Technique Indirect Microvessel Intraocular Pressure Galantamine business.industry Retinal Vessels Retinal medicine.disease Receptors Muscarinic eye diseases Rats Hypertonic saline Vasodilation Disease Models Animal medicine.anatomical_structure chemistry Retinal ganglion cell Acetylcholinesterase inhibitor Regional Blood Flow Anesthesia Acetylcholinesterase Autoradiography Ocular Hypertension Cholinesterase Inhibitors sense organs business Injections Intraperitoneal |
| Zdroj: | Investigative Opthalmology & Visual Science. 54:3171 |
| ISSN: | 1552-5783 |
| DOI: | 10.1167/iovs.12-11481 |
| Popis: | Purpose A clear correlation between vascular deficits and retinal ganglion cell (RGC) loss in glaucoma has not yet been established. The question arose as to whether there is loss of inner retinal vessels following intraocular pressure (IOP) increase and, if so, whether it occurs prior to, concomitantly with, or after RGC death. We also sought to establish whether galantamine, an acetylcholinesterase inhibitor that promotes RGC survival, can protect the retinal microvasculature and enhance blood flow in experimental glaucoma. Methods Ocular hypertension was induced in Brown Norway rats by injection of hypertonic saline into an episcleral vein. Retinas were processed for simultaneous visualization of the retinal microvasculature and RGCs in glaucomatous and control eyes. Retinal blood flow was examined by quantitative autoradiography using N-isopropyl-p-[(14)C]-iodoamphetamine. Vascular reactivity was further assessed using an in vitro retinal microvasculature preparation. Results Substantial loss of retinal capillaries was observed after induction of ocular hypertension. The onset of both microvasculature and RGC loss occurred early and proceeded at a similar rate for at least 5 weeks after the initial damage. Systemic administration of galantamine preserved microvasculature density and improved retinal blood flow in glaucomatous retinas. The vasoactive effects of galantamine on retinal microvessels occurred through activation of muscarinic acetylcholine receptors both in vitro and in vivo. Conclusions The onset and progression of microvessel and RGC loss are concomitant in experimental glaucoma, suggesting a tight codependence between these cellular compartments. Early interventions aimed to protect the retinal microvasculature and improve blood supply are likely to be beneficial for the treatment of glaucoma. |
| Databáze: | OpenAIRE |
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