Stimulants cocktail: Methylphenidate plus caffeine impairs memory and cognition and alters mitochondrial and oxidative status
| Autor: | Luciana Grazziotin Rossato-Grando, Amanda Carolina Cole Varela, Gessi Koakoski, Milena Fortuna, Mateus Timbola Mozzato, Suelen Mendonça Soares, Heloísa Helena de Alcantara Barcellos, Leonardo José Gil Barcellos, Victoria Costa Maffi, Aline Pompermaier, Natália Freddo |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Protein Carbonylation Physical exercise Oxidative phosphorylation Pharmacology medicine.disease_cause Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Cognition 0302 clinical medicine Caffeine mental disorders medicine Animals Cognitive Dysfunction Maze Learning Zebrafish Biological Psychiatry Memory Disorders business.industry Mitochondria 030227 psychiatry Oxidative Stress Mitochondrial respiratory chain Anxiogenic chemistry Methylphenidate Central Nervous System Stimulants Female business human activities Oxidative stress |
| Zdroj: | Progress in Neuro-Psychopharmacology and Biological Psychiatry. 106:110069 |
| ISSN: | 0278-5846 |
| DOI: | 10.1016/j.pnpbp.2020.110069 |
| Popis: | Methylphenidate (MPH) is a psychostimulant widely misused to increase wakefulness by drivers and students. Also, MPH can be found in dietary supplements in a clandestine manner aiming to burst performance of physical exercise practitioners. The abusive use of high doses of caffeine (CAF) in these contexts is equally already known. Here, we demonstrate the behavioral, oxidative and mitochondrial effects after acute exposure to high doses of MPH (80 mg/L) and CAF (150 mg/L), alone or associated (80 mg/L + 150 mg/L, respectively). We used zebrafish as animal model due to its high translational relevance. We evaluated the behavioral effects using the Novel Tank Test (NTT), Social Preference Test (SPT) and Y-maze Task and analyzed biomarkers of oxidative stress and activity of mitochondrial respiratory chain complexes. CAF alone caused a social impairment, increase in mitochondrial energy metabolism and nitric oxide. MPH alone induced antisocial behavior. Despite MPH inhibited lipid peroxidation, it increased protein carbonylation. The association of MPH + CAF presented memory impairment and anxiogenic behavior. In oxidative status, it inhibited lipid peroxidation, increased protein carbonylation and mitochondrial complex II, III and IV activity. Our results demonstrate that MPH and CAF alone negatively impact the typical behavioral of zebrafish. When associated, changes in cognition, memory, oxidative and mitochondrial status are more relevant. |
| Databáze: | OpenAIRE |
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